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Reproductive capacity of bacteroids in nodules of Trifolium repens L. and Glycine max (L.) Merr.

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Abstract

Reproductive growth of intracellular bacteria from isolated protoplasts in nodules of clover and soybean was directly investigated using a microchamber with visual and video recording. Differentiated bacteriods from clover nodules uniformly failed to reproduce. Such growth as occurred came from undifferentiated rhizobia from within the protoplast or extracellularly in the nodule. Plating investigation gave results in agreement with this conclusion. Osmoprotective media failed to secure the reproduction of differentiated clover bacteroids. Reproductive growth of bacteroids from protoplasts and crushed nodules of soybean was regularly observed in the microchamber and determined as proportionate colony-forming ability (CFA) on laboratory media. The CFA markedly increased with age of nodule and with the addition of nodule or root extract. The promoting effect of such extracts was reduced after heating for 60 min at 100°C, and lost completely after 20 min at 121°C. High osmolarity in the suspending and culture media was detrimental to bacteroid recovery.

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Abbreviations

BMM:

Bergersen's modified medium

B+m :

BMM with additional mannitol

CDB:

Chlamydomonas dilution buffer

PDB:

protoplast dilution buffer

PDB:

PDB without mannitol or sorbitol

RMM:

Rhizobium minimal medium

R+m :

RMM with mannitol instead of sucrose

YMA, YMB:

yeast mannitol agar and broth, respectively. For details, see Materials and methods

References

  1. Almon, L. (1933) Concerning the reproduction of bacteroids. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 87, 289–297

  2. Bergersen, F.J. (1968) The symbiotic state in legume root nodules; studies with the soybean system. Trans. IX Int. Congr. on Soil Science (Adelaide), vol. 2, pp. 49–63, Holmes, J.W., ed. Angus Robertson, Sydney

  3. Cao, Y.Z., Zhou, J.C., Chen, H.K. (1984) Differentiation and viability of nodule bacteria in host cells. Scientia Sinica, B. 27, 593–600

  4. Fred, E.B., Baldwin, J.L., McCoy, E. (1932) Root nodule bacteria and leguminous plants. Univ. of Wisconsin Press, Madison

  5. Gresshoff, P.M., Rolfe, B.G. (1978) Viability of Rhizobium bacteriods isolated from soybean nodule protoplasts. Planta 142, 329–333

  6. Gresshoff, P.M., Skotnicki, M.L., Eadie, J.F., Rolfe, B.G. (1977) Viability of Rhizobium trifolii bacteroids from clover root nodules. Plant Sci. Lett. 10, 299–304

  7. Sutton, W.D., Jepsen, N.M., Shaw, B.D. (1977) Changes in the number, viability, and amino-acid incorporating activity of Rhizobium bacteroids during lupin nodule development. Plant Physiol. 59, 741–744

  8. Sutton, W.D., Paterson, A.D. (1980) Effects of the plant host on the detergent sensitivity and viability of Rhizobium bacteroids. Planta 148, 287–292

  9. Sutton, W.D., Paterson, A.D. (1983) Further evidence for a plant host effect on Rhizobium bacteroid viability. Plant Sci. Lett. 30, 33–41

  10. Tsien, H.C., Cain, P.S., Schmidt, E.L. (1977) Viability of Rhizobium bacteroids. Appl. Environ Microbiol. 34, 854–856

  11. Vincent, J.M. (1970) A manual for the practical study of rootnodule bacteria. International Biological Programme Handbook No. 15. Blackwell, Oxford Edinburgh

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Zhou, J.C., Tchan, Y.T. & Vincent, J.M. Reproductive capacity of bacteroids in nodules of Trifolium repens L. and Glycine max (L.) Merr.. Planta 163, 473–482 (1985). https://doi.org/10.1007/BF00392704

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Key words

  • Bacteroid (reproductive capacity)
  • Bradyrhizobium
  • Glycine (bacteroids)
  • Nodule extract
  • Nodule protoplast
  • Rhizobium
  • Trifolium