Marine Biology

, Volume 98, Issue 1, pp 143–155

Nitrogen fixation on a coral reef

  • A. W. D. Larkum
  • I. R. Kennedy
  • W. J. Muller


Acetylene reduction was used to assess nitrogen fixation on all major substrates at all major areas over a period of 1 to 6 yr (1980–1986) at One Tree Reef (southern Great Barrier Reef). Experiments using 15N2 gave a ratio of 3.45:1.0 for C2H2 reduced:N2 fixed. Acetylene reduction was largely light-dependent, saturated at 0.15 ml C2H2 per ml seawater, and linear over 6 h. High fixation was associated with two emergent cyanophyte associations, Calothrix crustacea and Scytonema hofmannii, of limited distribution. Subtidally, the major contribution to nitrogen fixation came from well-grazed limestone substrates with an epilithic algal community in the reef flat and patch reefs (3 to 15 nmol C2H4 cm-2 h-1). Similar substrates from the outer reef slope showed lower rates. Nitrogen fixation on beach rock, intertidal coral rubble, reef crest and lagoon sand was relatively small (0.3 to 1.0 nmol C2H4 cm-2 h-1). Seasonal changes in light-saturated rates were small, with slight reduction only in winter. Rates are also reported for experimental coral blocks (13 to 39 nmol cm-2 h-1) and for branching coral inside and outside territories of gardening damselfish (3 to 28 nmol cm-2 h-1). This work supports the hypothesis that the high nitrogen fixation on the reef flat and patch reefs of the lagoon (34 to 68 kg N ha-1 yr-1) is because these subtidal areas support highly disturbed communities with the greatest abundance of nitrogen-fixing cyanophyte algae. It is calculated from a budget of all areas that One Tree Reef has an annual nitrogen fixation rate of 8 to 16 kg N ha-1 yr-1.


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Literature cited

  1. Andrews, J. C., Muller H. (1983). Space time variability of nutrients in a lagoonal patch reef. Limnol. Oceanogr. 28: 215–227Google Scholar
  2. Bergersen, F. J. (1980). Measurements of nitrogen fixation by direct means. In: F. J. Bergersen (ed.) Methods for evaluating biological nitrogen fixation. Wiley-Interscience, Chichester, p. 65–110Google Scholar
  3. Bergersen, F. J., Turner, G. L. (1983). An evaluation of 15N methods for estimating nitrogen fixation in subterranean clover-perennial grass pasture. Aust. J. agric. Res. 34: 391–401Google Scholar
  4. Borowitzka, M. A., Day, R., Larkum A. W. D. (1983). The importance of production by turf and crustose algal communities in One Tree Lagoon. In: Baker, J., Carter R. M., Sammarco P. W., Stark, K. P. (eds.) Proceedings of the First Great Barrier Reef Conference JCU Press, Townsville, p. 259–292Google Scholar
  5. Borowitzka, M. A., Larkum, A. W. D., Borowitzka, L. J. (1978). A preliminary study of algal turf communities of a shallow coral reef lagoon using an artificial substratum. Aquat. Bot. 5: 365–381Google Scholar
  6. Burris, R. H. (1976). Nitrogen fixation by blue-green algae of Lizard Island area of the Great Barrier Reef. Aust. J. Pl. Physiol. 3: 41–51Google Scholar
  7. Burris, R. H., Wilson, P. W. (1957). Methods for measurement of nitrogen fixation. Meth. Enzym. 4: 355–366Google Scholar
  8. Capone, D. G., Taylor, D. L., Taylor, B. F. (1977). Nitrogen fixation (acetylene reduction) associated with macroalgae in a coral-reef community in the Bahamas. Mar. Biol. 40: 29–32Google Scholar
  9. Cox, G. C., Hiller, R. G., Larkum, A. W. D. (1985). An unusual cyanophyte, containing phycourobilin and symbiotic with sponges and ascidians. Mar. Biol. 89: 149–163Google Scholar
  10. Crossland, C. J., Barnes, D. J. (1976). Acetylene reduction by coral skeletons. Limnol. Oceanogr. 21: 153–155Google Scholar
  11. Crossland, C. J., Barnes, D. J. (1983). Dissolved nutrients and organic particulates in water flowing over coral reefs at Lizard Island. Aust. J. mar. Freshwat. Res. 34: 835–844Google Scholar
  12. Dahl, A. L. (1973). Surface area in ecological analysis: quantitation of benthic coral reef algae. Mar. Biol. 23: 239–249Google Scholar
  13. Flett, R. J., Hamilton, R. D., Campbell, N. E. R. (1976). Aquatic acetylene-reduction techniques: solutions to several problems. Can. J. Microbiol. 22: 43–51Google Scholar
  14. Goering, J. J. (1983). Marine denitrification. In: Golterman, H. L. (ed.) Denitrification in the nitrogen cycle. Plenum Press, New York, p. 191–224Google Scholar
  15. Goldman, B., Talbot, F. H. (1976). Aspects of the ecology of coral reef fishes. In: Jones, O. A., Endean, R. (eds.) Biology and geology of coral reefs, Vol. 3. Academic Press, New York, p. 125–154Google Scholar
  16. Goldner, L. L. (1980). Nitrogen fixation (acetylene reduction) in shallow water Bahamian environments. Bull. mar. Sci. 30: 444–454Google Scholar
  17. Hanson, R. B., Gundersen, K. (1977). Relationship between nitrogen fixation (acetylene reduction) and the C:N ratio in a polluted coral reef ecosystem, Kaneohe Bay, Hawaii, Estuar. cstl mar. Sci. 5: 437–444Google Scholar
  18. Hatcher, A. I., Frith, C. A. (1985). The control of nitrate and ammonium concentrations in a coral reef lagoon. Coral Reefs 4: 101–110Google Scholar
  19. Hatcher, A. I., Hatcher, B. G. (1981). Seasonal and spatial variation in dissolved inorganic nitrogen in One Tree Reef Lagoon. Proc. 4th. int. Symp. coral Reefs 1: 419–424 [Gomez, E. D. et al. (eds.) Marine Sciences Centre, University of the Philippines, Quezon City]Google Scholar
  20. Hatcher, B. G. (1981). The interaction between algae and grazers on a coral reef. Ph.D. dissertation, University of Sydney, AustraliaGoogle Scholar
  21. Hatcher, B. G., Larkum, A. W. D. (1983). Ah experimental analysis of factors controlling the standing crop of the epilithic algal community on a coral reef. J. exp mar. Biol. Ecol. 69: 61–84Google Scholar
  22. Humm, H. J., Wicks, S. R. (1980). Introduction and guide to the marine bluegreen algae. Wiley-Interscience, New YorkGoogle Scholar
  23. International Critical Tables (1928). Vol. 3. McGraw Hill, New York, p.260 and p. 280Google Scholar
  24. Johannes, R. E., Alberts, J., D'Elia, C., Kinzie, R. A., Pomeroy, L. R., Sottile, S., Wiebe, W., Marsh, J. R. Jr., Helfrich, P., Maqragos, J., Meyer, J., Smith, S., Crabtree, D., Roth, A., McCloskey, L. R., Betzer, S., Marshall, N., Pilson, M. E. Q., Telek, G., Clutter, R. I., Dupaul, W. D., Webb, K. L., Wells, J. M. Jr. (1972). The metabolism of some coral reef communities: a team study of nutrient and energy flux at Eniwetok. BioSci. 22: 541–543Google Scholar
  25. Kallas, T., Rippka, R., Coursin, T., Rebiere, M-C., Tandeau de Marsac, N., Cohen-Bazire, G. (1983). Aerobic nitrogen fixation by nonheterocystous cyanobacteria. In: Papageorgiou, G. C., Packer, L. (eds.) Photosynthetic prokaryotes. Elsevier, Amsterdam, p. 287–302Google Scholar
  26. Kinsey, D. W., Davies, P. J. (1979). Carbon turnover, calcification and growth in coral reefs. In: Trudinger, P. A., Swaine, D. J. (eds.) Biogeochemical cycling of mineral forming elements. Studies in environmental science, Vol. 3. Elsevier, Amsterdam, p. 287–302Google Scholar
  27. Kinsey, D. W., Domm, A. (1974). Effects of fertilization on a coral reef environment-primary production studies. Proc. 2nd int. Symp. coral Reefs. 1: 49–66 [Cameron, A. M. et al. (eds.) Great Barrier Reef Committee, Brisbane]Google Scholar
  28. Larkum, A. W. D. (1988). High rates of nitrogen fixation on coral skeletons after predation by the crown of thorns starfish Acanthaster planci. Mar. Biol. 97: 503–506Google Scholar
  29. Larkum, A. W. D., Cox G. C., Hiller, R. G., Parry, D. L., Dibbayawan, T. P. (1987). Filamentous cyanophytes containing phycourobilin and in symbiosis with sponges and an ascidian of coral reefs. Mar. Biol. 95: 1–13Google Scholar
  30. Lewis, J. B. (1977). Processes of organic production on coral reefs. Biol. Rev. 52: 305–347Google Scholar
  31. Mague, T. H., Holm-Hansen, O. (1975). Nitrogen fixation on a coral reef. Phycologia 14: 87–92Google Scholar
  32. Paerl, H. W., Webb K. L., Baker, J., Wiebe, W. J. (1981). Nitrogen fixation in waters. In: Broughton, W. J. (ed.) Nitrogen fixation, Vol. I. Ecology. Clarendon Press, Oxford, p. 193–240Google Scholar
  33. Peterson, R. B., Burris, R. H. (1976). Conversion of acetylene reduction rates to nitrogen fixation rates in natural populations of blue green algae. Analyt. Biochem. 73: 404–410Google Scholar
  34. Postgate, J. R. (1982). The fundamentals of nitrogen fixation. Cambridge University Press, CambridgeGoogle Scholar
  35. Potts, M., Whitton, B. A. (1977). Nitrogen fixation by blue-green algal communities in the intertidal zone of the lagoon of Aldabra Atoll. Oecologia 27: 275–283Google Scholar
  36. Ryther, J. (1969). Potential productivity of the sea. Science, N.Y. 130: 602–608Google Scholar
  37. Sharp, J. H. (1983). The distribution of inorganic nitrogen and dissolved and particulate organic nitrogen in the sea. In: Carpenter, E. J., Capone, D. G. (eds.) Nitrogen in the marine environment. Academic Press, New York, p 1–35Google Scholar
  38. Stewart, W. D. P., Fitzgerald, G. P., Burris, R. H. (1968). Acetylene reduction by nitrogen fixing blue-green algae. Arch. Mikrobiol. 62: 336–348Google Scholar
  39. Underwood, A. J. (1981). Techniques of analysis of variance in experimental marine biology and ecology. Oceanogr. mar. Biol. A. Rev. 18: 513–605Google Scholar
  40. Wanders, J. B. W. (1976). The role of benthic algae in the shallow reef of Curaçao (Netherland Antilles). I: Primary productivity in the coral reef. Aquat. Bot. 2: 235–270Google Scholar
  41. Wanders, J. B. W. (1977). The role of benthic algae in the shallow reef of Curaçao (Netherlands Antilles). III. The significance of grazing. Aquat. Bot. 3: 357–390Google Scholar
  42. Webb, K. L., Paul, W. D., Wiebe, W., Sottile, W., Johannes, R. E. (1975). Enewetak (Eniwetok) Atoll: aspects of the nitrogen cycle on a coral reef. Limnol. Oceanogr. 20: 198–210Google Scholar
  43. Wiebe, W. J., Johannes, R. E., Webb, K. L., (1975). Nitrogen fixation in a coral reef community. Science, N.Y. 188: 257–259Google Scholar
  44. Wilkinson, C. R., Sammarco, P. W. (1983). Effects of fish grazing and damselfish territoriality on coral reef algae. II. Nitrogen fixation. Mar. Ecol. Prog. Ser. 13: 15–19Google Scholar
  45. Wilkinson, C. R., Sammarco, P. W., Trott, L. A. (1985). Seasonal and fish grazing effects on rates of nitrogen fixation on coral reefs (Great Barrier Reef). Proc. 5th int. coral Reef Congr. 61–65 [Gabrie, C. et al. (eds.) Antenne Museum-EPHE, Moorea, French Polynesia]Google Scholar
  46. Wilkinson, C. R., Williams, D. McB., Sammarco, P. W., Hogg, R. W., Trott, L. A. (1984). Rates of nitrogen fixation on coral reefs across the continental shelf of the central Great Barrier Reef. Mar. Biol. 80: 255–262Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • A. W. D. Larkum
    • 1
  • I. R. Kennedy
    • 2
  • W. J. Muller
    • 3
  1. 1.School of Biological SciencesUniversity of SydneySydneyAustralia
  2. 2.Department of Agricultural Chemistry, School of AgricultureUniversity of SydneySydneyAustralia
  3. 3.CSIRO Division of Mathematics and StatisticsCanberraAustralia

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