Marine Biology

, Volume 91, Issue 4, pp 449–459 | Cite as

Yolk resorption, onset of feeding and survival potential of larvae of three tropical marine fish species reared in the hatchery

  • T. Bagarinao


This paper provides basic early life-history information on milkfish (Chanos chanos), seabass (Lates calcarifer) and rabbitfish (Siganus guttatus) which may explain in part the observed differences in their survival performance in the hatchery. Egg size, larval size, amount of yolk and oil reserves and mouth size are all greater in milkfish than in seabass, and greater in the latter than in rabbitfish. During the first 24 h after hatching, rabbitfish larvae grow much faster than milkfish and seabass larvae at similar ambient temperatures (range 26°–30°C, mean about 28°C). The eyes become fully pigmented and the mouths open earlier in seabass and rabbitfish (32–36 h from hatching) than in milkfish (54 h). Seabass larvae learn to feed the earliest. Yolk is completely resorbed at 120 h from hatching in milkfish, and yolk plus oil at 120 h in seabass and 72 h in rabbitfish at 26° to 30°C. Milkfish and seabass larvae have more time than rabbitfish to initiate external feeding before the endogenous reserves are completely resorbed. Delayed feeding experiments showed that 50% of unfed milkfish larvae die at 78 h and all die at 150 h from hatching. Milkfish larvae fed within 54 to 78 h after hatching had improved survival times: 50% mortality occurred at 96 to 120 h, and 10 to 13% survived beyond 150 h. Unfed seabass larvae all died at 144 h, while 6 to 13% of those fed within 32 to 56 h after hatching survived beyond 144 h and well into the subsequent weeks. Unfed rabbitfish larvae all died at 88 h, while 7 to 12% of those fed within 32 to 56 h after hatching survived beyond 88 h. A delay in initial feeding of more than 24 h after eye pigmentation and opening of the mouth may be fatal for all three species.


Ambient Temperature Survival Time Fish Species Marine Fish Survival Potential 
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Literature cited

  1. Akatsu, S., K. M. Al-Abdul-Elah and S. K. Teng: Effects of salinity and water temperature on the survival and growth of brown-spotted grouper larvae (Epinephelus tauvina, Serranidae). J. World maric. Soc. 14, 624–635 (1983)Google Scholar
  2. Anonymous: Report of training course on seabass spawning and larval rearing, 105 pp. Manila: South China Sea Fisheries Development and Coordinating Programme 1982Google Scholar
  3. Avila, E. M.: Hormone-induced spawning and embryonic development of the rabbitfish, Siganus vermiculatus (Pisces: Siganidae). Philipp. Sci. 21, 75–108 (1984)Google Scholar
  4. Bagenal, T. B.: The interrelation of the size of fish eggs, the date of spawning and the production cycle. J. Fish Biol. 3, 207–219 (1971)Google Scholar
  5. Blaxter, J. H. S.: Development: eggs and larvae. In: Fish physiology 3, pp 177–252. Ed. by W. S. Hoar and D. J. Randall. New York: Academic Press 1969Google Scholar
  6. Blaxter, J. H. S. (Ed.): The early life history of fish, 765 pp. New York: Springer-Verlag 1974Google Scholar
  7. Blaxter, J. H. S. and G. Hempel: The influence of egg size on herring larvae (Clupea harengus L.). J. Cons. int. Explor. Mer 28, 211–244 (1963)Google Scholar
  8. Blaxter, J. H. S. and M. E. Staines: Food searching potential in marine fish larvae. In: 4th Eur. Mar. Biol. Symp., pp 467–485. Ed. by D. J. Crisp. Cambridge: Cambridge University Press 1971Google Scholar
  9. Bryan, P. G. and B. B. Madraisau: Larval rearing and development of Siganus lineatus (Pisces:Siganidae) from hatching through metamorphosis. Aquaculture 10, 243–252 (1977)Google Scholar
  10. Burgan, B. G. and K. A. Zcelecsky: Induced spawning and early development of the rabbitfish, Siganus argenteus (Quoy and Gaimard) in the Philippines. Silliman J. 26, 163–171 (1979)Google Scholar
  11. Chaudhuri, H., J. V. Juario, J. H. Primavera, R. Samson and R. Mateo: Observations on artificial fertilization of eggs and the embryonic and larval development of milkfish, Chanos chanos (Forskal). Aquaculture 13, 95–113 (1978)Google Scholar
  12. Chen, F. Y., M. Chow, T. M. Chao and R. Lim: Artificial spawning and larval rearing of the grouper, Epinephelus tauvina (Forskal) in Singapore. Singapore J. Pri. Ind. 5, 1–21 (1977)Google Scholar
  13. Cushing, D. H.: The production cycle and the numbers of marine fish. Symp. Zool. Soc. Lond. 29, 213–232 (1972)Google Scholar
  14. Fukusho, K.: Studies on fry production of Japanese striped knifejaw, Oplegnathus fasciatus, with special reference to feeding ecology and mass culture of food organisms. Spec. Report Nagasaki Pref. Inst. Fish. No. 6 (in Japanese with English summary) 1979Google Scholar
  15. Fujita, S. and M. Ueno: On the development of the egg and prelarval stages of Siganus fuscescens (Houttuyn) by artificial insemination. Jap. J. Ichthyol. 3, 129–132 (1954)Google Scholar
  16. Houde, E. D.: Development and early life history of the northern sennet, Sphyraena borealis (Pisces: Sphyraenidae) reared in the laboratory. Fish. Bull., U.S. 70, 185–195 (1972)Google Scholar
  17. Houde, E. D.: Effects of delayed feeding on growth and survival of larvae of three species of subtropical marine fishes. Mar. Biol. 26, 271–285 (1974)Google Scholar
  18. Houde, E. D. and B. J. Palko: Laboratory rearing of the clupeid fish Harengula pensacolae from fertilized eggs. Mar. Biol. 5, 354–358 (1970)Google Scholar
  19. Houde, E. D., S. A. Berkeley, J. J. Klinovsky and R. C. Schekter: Culture of the larvae of the white mullet, Mugil curema Valenciennes. Aquaculture 8, 365–370 (1976)Google Scholar
  20. Hunter, J. R.: Feeding ecology and predation of marine fish larvae. In: Marine fish larvae, pp 34–77. Ed. by R. Lasker. Seattle: Washington Sea Grant Program 1981Google Scholar
  21. Hussain, N. A. and M. Higuchi: Larval rearing and development of the brown-spotted grouper, Epinephelus tauvina (Forskål). Aquaculture 19, 339–350 (1980)Google Scholar
  22. Hussain, N., S. Akatsu and C. El-Zahr: Spawning, egg and early larval development and growth of Acanthopagrus cuvieri (Sparidae). Aquaculture 22, 125–136 (1981)Google Scholar
  23. Hussain, N., M. Saif and M. Ukawa: On the culture of Epinephelus tauvina (Forskål). Kuwait Inst. Scient. Res. 14 pp. 1975Google Scholar
  24. Juario, J. V., M. N. Duray, V. M. Duray, J. F. Nacario and J. M. E. Almendras: Induced breeding and larval rearing experiments with milkfish, Chanos chanos (Forskål) in the Philippines. Aquaculture 36, 61–70 (1984)Google Scholar
  25. Juario, J. V., M. N. Duray, V. M. Duray, J. F. Nacario and J. M. E. Almendras: Breeding and larval rearing of the rabbitfish, Siganus guttatus (Bloch). Aquaculture 44, 91–101 (1985)Google Scholar
  26. Kosutarak, P. and T. Watanabe: Growth and survival of newly hatched larvae of seabass, Lates calcarifer, in starved condition. In: Report of Thailand and Japan Joint Coastal Aquaculture Research Project No. 1, 81–82. Japan International Cooperation Agency 1984Google Scholar
  27. Kumagai, S.: The ecological aspects of milkfish fry occurrence particularly in the Philippines. In: Advances in milkfish biology and culture, pp 53–68. Ed. by J. V. Juario, R. P. Ferraris and L. V. Benitez. Manila: Island Publishing House, Inc. 1984Google Scholar
  28. Kuo, C. M., Z. H. Shehadeh and K. K. Milisen: A preliminary report on the development, growth and survival of laboratory-reared larvae of the grey mullet, Mugil cephalus L. J. Fish. Biol. 5, 459–470 (1973)Google Scholar
  29. Lam, T. J.: Siganids: their biology and mariculture potential. Aquaculture 3, 325–354 (1974)Google Scholar
  30. Lasker, R. and K. Sherman (Eds.): The early life history of fish: recent studies, 607 pp. Rapp. P.-v. Cons. int. Explor. Mer 178 (1981)Google Scholar
  31. Laurence, G. C.: Comparative growth, respiration and delayed feeding abilities of larval cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) as influenced by temperature during laboratory studies. Mar. Biol. 50, 1–7 (1978)Google Scholar
  32. Liao, I. C., J. V. Juario, S. Kumagai, H. Nakajima, M. Natividad and P. Buri: On the induced spawning and larval rearing of milkfish Chanos chanos (Forskal). Aquaculture 18, 75–93 (1979)Google Scholar
  33. Manacop, P. R.: The artificial propagation of dangit, Amphacanthus oramin (Bloch and Schneider). Philipp, J. Sci. 62, 229–237 (1937)Google Scholar
  34. Maneewongsa, S. and T. Tattanon: Nature of eggs, larvae and juveniles of the seabass. In: Report of the training course on seabass spawning and larval rearing, pp 22–24. Manila: South China Sea Fisheries Development and Coordinating Programme 1982Google Scholar
  35. May, R. C.: Effects of delayed initial feeding on larvae of the grunion, Leuresthes tenuis (Ayres). Fish. Bull. 69, 411–425 (1971)Google Scholar
  36. May, R. C.: Larval mortality in marine fishes and the critical period concept. In: The early life history of fish, pp 3–19. Ed. by J. H. S. Blaxter. New York: Springer Verlag 1974Google Scholar
  37. May, R. C., D. Popper and J. P. McVey: Rearing and larval development of Siganus canaliculatus (Park) (Pisces:Siganidae). Micronesica 10, 285–298 (1974)Google Scholar
  38. McGurk, M. D.: Effects of delayed feeding and temperature on the age of irreversible starvation and on the rates of growth and mortality of Pacific herring larvae. Mar. Biol. 84, 13–26 (1984)Google Scholar
  39. Moser, H. G., W. J. Richards, D. M. Cohen, M. P. Fahay, A. W. Kendall, Jr. and S. L. Richardson (Eds.): Ontogeny and systematics of fishes, 760 pp. Spec. Publ. No. 1, American Society of Ichthyologists and Herpetologists 1984Google Scholar
  40. Popper, D., H. Gordin and G. W. Kissil: Fertilization and hatching of the rabbitfish Siganus rivulatus. Aquaculture 2, 37–44 (1973)Google Scholar
  41. Popper, D., R. C. May and T. Lichatowich: An experiment in rearing larval Siganus vermiculatus (Valenciennes) and some observations on its spawning cycle. Aquaculture 7, 281–290 (1976)Google Scholar
  42. Popper, D., R. Pitt and Y. Zohar: Experiments on the artificial propagation of Red Sea siganids and some notes on their reproduction in nature. Aquaculture 16, 177–181 (1979)Google Scholar
  43. Roberts, D. E., L. A. Morey III, G. E. Henderson and K. R. Halscott: The effects of delayed feeding, stocking density and food density on the survival, growth and production of larval red drum (Sciaenops ocellata). Proc. 9th Ann. Meet. World Maric. Soc., 333–343 (1978)Google Scholar
  44. Shirota, A.: Studies on the mouth size of fish larvae. Bull. Jpn. Soc. Sci. Fish. 36, 353–368 (1970)Google Scholar
  45. Soh, C. L. and T. J. Lam: Induced breeding and early development of the rabbitfish Siganus oramin (Schneider), pp 49–56. Proc. Symp. Biol. Res. Nat. Dev., Mal. Nat. Soc. 1973Google Scholar
  46. Soletchnik, P.: Aspects of nutrition and reproduction in Siganus guttatus, with emphasis on applications to aquaculture, 75 pp. Terminal report to the SEAFDEC Aquaculture Dept. 1983Google Scholar
  47. Spectorova, L. V., T. M. Aronovich, S. I. Doroshev and V. P. Popova: Artificial rearing of the Black Sea turbot larvae (Scophthalmus maeoticus). Aquaculture 4, 329–340 (1974)Google Scholar
  48. Theilacker, G. and K. Dorsey: Larval fish diversity, a summary of laboratory and field research. In: Workshop on the effects of environmental variation on the survival of larval pelagic fishes, G. Sharp (Rapp.). UNESCO IOC Workshop Report 28, 105–142 (1980)Google Scholar
  49. Ukawa, M., M. Higuchi and S. Mito: Spawning habits and early life history of a serranid fish, Epinephelus akaara (Temminck et Schlegel). Jap. J. Ichthyol. 13, 156–161 (1966)Google Scholar
  50. Vanstone, W. E., L. B. Tiro, Jr., A. C. Villaluz, D. C. Ramsingh, S. Kumagai, P. J. Duldoco, M. M. L. Barnes and C. E. Due nas: Breeding and larval rearing of the milkfish Chanos chanos. In: Induced spawning, artificial fertilization of eggs and larval rearing of the milkfish Chanos chanos (Forskal) in the Philippines. Tech. Report No. 1, 3–17. SEAFDEC Aquaculture Department 1977Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • T. Bagarinao
    • 1
  1. 1.Aquaculture DepartmentSoutheast Asian Fisheries Development CenterIloilo CityPhilippines

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