Membrane-potential changes in vacuoles isolated from storage roots of red beet (Beta vulgaris L.)
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Abstract
The membrane potential in vacuoles isolated from storage roots of red beet (Beta vulgaris L.) has been studied by following changes in the fluorescence of the dye 3,3′-diethylthiodicarbocyanine iodide, and by determining the uptake of the lipophilic triphenylmethylphosphonium cation. The vacuoles have a membrane potential, internal negative, which is estimated to be around-60 mV. These potentials become less negative by nearly 10 mV on addition of ATP. This ATP-dependent depolarisation is inhibited by the protonophore carbonylcyanide p-trifluoromethoxyphenylhydrazone and by the ATPase inhibitors, N,N′-dicyclohexylcarbodiimide and trimethyltin chloride, but it is largely insensitive to sodium orthovanadate. Fusicoccin had no significant effect on the isolated vacuoles, but its addition to excised tissue caused a hyperpolarisation of the cells measured using a microelectrode.
Key words
ATPase Beta (membrane potential) Membrane potential Fusicoccin Vacuole (membrane potential)Abbreviations
- DCCD
N,N′-dicyclohexylcarbodiimide
- DiS-C2-(5)
3,3′-diethylthiodicarbocyanine iodide
- FCCP
carbonylcyanide p-trifluoromethoxyphenylhydrazone
- TPMP+
triphenylmethylphosphonium ion
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