Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Cytoplasmic free calcium in Riccia fluitans L. and Zea mays L.: Interaction of Ca2+ and pH?

  • 81 Accesses

  • 166 Citations

Abstract

In cells of Zea mays (root hairs, coleoptiles) and Riccia fluitans (rhizoids, thalli) intracellular Ca2+ and pH have been measured with double-barrelled microelectrodes. Free Ca2+ activities of 109–187 nM (Riccia rhizoids), 94–160 nM (Riccia thalli), 145–231 nM (Zea root hairs), 84–143 nM (Zea coleoptiles) were found, and therefore identified as cytoplasmic. In a few cases (Riccia rhizoids), free Ca2+ was in the lower millimolar range (2.3±0.8 mM). A change in external Ca2+ from 0.1 to 10 mM caused an initial and short transient increase in cytoplasmic free Ca2+ which finally levelled off at about 0.2 pCa unit below the control, whereas in the presence of cyanide the Ca2+ activity returned to the control level. It is suggested that this behaviour is indicative of active cellular Ca2+ regulation, and since it is energy-dependent, may involve a Ca2+-ATPase. Acidification of the cytoplasmic pH and alkalinization of the vacuolar pH lead to a simultaneous increase in cytoplasmic free Ca2+, while alkalinization of pHc decreased the Ca2+ activity. Since this is true for such remote organisms as Riccia and Zea, it may be concluded that regulation of cytoplasmic pH and free Ca2+ are interrelated. It is further concluded that double-barrelled microelectrodes are useful tools for investigations of intracellular ion activities in plant cells.

This is a preview of subscription content, log in to check access.

Abbreviations

Ψm, ΔΨm :

membrane potential difference, changes thereof

PVC:

polyvinylchloride

References

  1. Ammann, D. (1986) Ion-selective microelectrodes. Springer, Berlin Heidelberg New York Tokyo

  2. Ammann, D., Bührer, T., Schefer, U., Müller, M., Simon, W. (1987) Intracellular neutral carrier-based Ca2+ microelectrodes with subnanomolar detection limit. Pflügers Arch. 409, 223–228

  3. Bertl, A., Felle, H., Bentrup, F.W. (1984) Amine transport in Riccia flutans. Cytoplasmic and vacuolar pH recorded by a pH-sensitive microelectrode. Plant Physiol. 76, 75–78

  4. Blinks, J.R., Wier, W.G., Hess, P., Prendergast, F.G. (1982) Measurement of Ca2+ concentrations in living cells. Prog. Biophys. Mol. Biol. 40, 1–114

  5. Brownlee, C., Wood, J.W. (1986) A gradient of cytoplasmic free calcium in growing rhizoid cells of Fucus serratus. Nature 320, 624–628

  6. Buckhout, T.J. (1983) ATP-dependent Ca2+ transport in endoplasmatic reticulum isolated from roots of Lepidium sativum. Planta 159, 84–90

  7. Carafoli, E. (1987) Intracellular calcium homeostasis. Annu Rev. Biochem. 56, 395–433

  8. Dieter, P., Marmé, D. (1980) Calmodulin activation of plant microsomal Ca2+ uptake. Proc. Natl. Acad. Sci. USA 77, 7311–7314

  9. Felle, H. (1981) A study of the current-voltage relationships of electrogenic active and passive membrane elements in Riccia fluitans. Biochim. Biophys. Acta 646, 151–160

  10. Felle, H. (1984) Steady state current-voltage characteristics of amino acid transport in rhizoid cells of Riccia fluitans. Is the carrier negatively charged? Biochim. Biophys. Acta 772, 307–312

  11. Felle, H. (1987) Proton transport and pH control in Sinapis alba root hairs. A study carried out with double barrelled pH microelectrodes. J. Exp. Bot. 38, 340–354

  12. Felle, H. (1988) Auxin causes oscillations of cytosolic free calcium and pH in Zea mays coleoptiles. Planta 174, 495–499

  13. Felle, H., Bertl, A. (1986) The fabrication of H+-selective liquid-membrane micro-electrodes for use in plant cells. J. Exp. Bot. 37, 1416–1428

  14. Felle, H., Brummer, B., Bertl, A., Parish, R.W. (1986) Indole-3-acetic acid and fusicoccin cause sytosolic acidification of corn coleoptile cells. Proc. Natl. Acad. Sci., USA 83, 8992–8995

  15. Gilroy, S., Hughes, W.A., Trewavas, A.J. (1986) The measurement of intracellular calcium levels in protoplasts from higher plant cells. FEBS Lett. 199, 217–221

  16. Gilroy, S., Hughes, W.A., Trewavas, A.J. (1987) Calmodulin antagonists increase free cytosolic calcium levels in plant protoplasts in vivo. FEBS Lett. 212, 133–137

  17. Hepler, P.H., Wayne, R.O. (1985) Calcium and plant development. Annu. Rev. Plant Physiol. 36, 397–493

  18. Johannes, E., Felle, H. (1987) Implications for cytoplasmic pH, protonmotive force, and amino-acid transport across the plasmalemma of Riccia fluitans. Planta 172, 53–59

  19. Miller, A.J., Sanders, D. (1987) Depletion of cytosolic free calcium induced by photosynthesis. Nature 326, 397–400

  20. Rasi-Caldogno, F., Pugliarello, M.C., DeMichelis, M.I. (1987) The Ca2+-transport ATPase of plant plasma membrane catalyses a nH+/Ca2+ exchange. Plant Physiol. 83, 994–1000

  21. Rasmussen, H., Barrett, P.Q. (1984) Calcium messenger system: An integrated view. Physiol. Rev. 64, 938–984

  22. Reid, R.J., Field, L.D., Pitman, M.G. (1985) Effects of external pH, fusicoccin and butyrate on the cytoplasmic pH in barley root tips measured by 31P-nuclear magnetic resonance spectroscopy. Planta 160, 341–347

  23. Sanders, D., Hansen, U.-P., Slayman, C.L. (1981) Role of the plasma membrane proton pump in pH regulation in nonanimal cells. Proc. Natl. Acad. Sci. USA 78, 5903–5907

  24. Sanders, D., Slayman, C.L. (1982) Control of intracellular pH. Predominant role of oxidative metabolism not proton transport, in the eucaryotic microorganism Neurospora. J. Gen. Physiol. 80, 377–402

  25. Schumaker, K.S., Sze, H. (1987) Inositol-1,4,5-triphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots. J. Biol. Chem. 262, 3944–3946

  26. Tsien, R.J., Rink, T.J. (1980) Neutral carrier ion-selective microelectrodes for measurement of intracellular free calcium. Biochim. Biophys. Acta 599, 623–638

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Felle, H. Cytoplasmic free calcium in Riccia fluitans L. and Zea mays L.: Interaction of Ca2+ and pH?. Planta 176, 248–255 (1988). https://doi.org/10.1007/BF00392452

Download citation

Key words

  • Calcium (free, and pH)
  • Cytoplasm (free Ca2+, pH)
  • Microelectrode, double-barrelled, Ca2+
  • pH(interaction with Ca2+)
  • Riccia
  • Zea (free Ca2+ and pH)