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Marine Biology

, Volume 82, Issue 3, pp 225–232 | Cite as

Photosynthesis-irradiance responses and photosynthetic periodicity in the sea anemone Aiptasia pulchella and its zooxanthellae

  • G. Muller-Parker
Article

Abstract

Sea anemones (Aiptasia pulchella) containing zooxanthellae (Symbiodinium microadriaticum) were maintained in a long-term laboratory culture on a 12 h light (100 μE m-2 s-1):12 h dark cycle. Photosynthetic oxygen production was measured for the symbiotic association and for freshlyisolated zooxanthellae. Light utilization efficiencies (α) were similar for both sets of zooxanthellae, suggesting negligible shading of zooxanthellae by animal tissue in this association. Whereas freshly-isolated zooxanthellae were photoinhibited at high irradiances (800 to 1 800 μE m-2 s-1), zooxanthellae in the host continued to function at photosynthetic capacity. Time of day may influence photosynthetic measurements in symbiotic organisms, as it was found that photosynthesis in A. pulchella followed a diel periodicity at both light-saturating (1 200 μE m-2 s-1) and subsaturating (150 μE m-2 s-1) irradiances. There was a peak period of photosynthesis between 12.00 and 14.00 hrs. Light stimulated dark respiration rates of A. pulchella. Dark respiration of sea anemones increased somewhat towards the end of the light cycle and was always greater after exposure to high irradiances.

Keywords

Photosynthesis Photosynthetic Capacity Utilization Efficiency High Irradiance Peak Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature cited

  1. Barnes, D. J. and D. L. Taylor: In situ studies of calcification and photosynthetic carbon fixation in the coral Montastrea annularis. Helgoländer wiss. Meeresunters. 24, 284–291 (1973)Google Scholar
  2. Cary, L. R.: A study of pedal laceration in actinians. Biol. Bull. mar. biol. Lab., Woods Hole 20, 81–108 (1911)Google Scholar
  3. Chalker, B. E.: Daily variation in the calcification capacity of Acropora cervicornis. Proc. 3rd int. Symp. coral Reefs 2, 417–423 (1977). (Ed. by D. L. Taylor. Miami: School of Marine and Atmospheric Sciences, University of Miami)Google Scholar
  4. Chalker, B. E. and D. L. Taylor: Rhythmic variations in calcification and photosynthesis associated with the coral Acropora cervicornis (Lamarck). Proc. R. Soc. (Ser. B) 201, 179–189 (1978)Google Scholar
  5. Chang, S. S., B. B. Prézelin and R. K. Trench: Mechanisms of photoadaptation in three strains of the symbiotic dinoflagellate Symbiodinium microadriaticum. Mar. Biol. 76, 219–229 (1983)Google Scholar
  6. Crossland, C. J. and D. J. Barnes: Gas-exchange studies with the staghorn coral Acropora acuminata and its zooxanthellae. Mar. Biol. 40, 185–194 (1977)Google Scholar
  7. Dustan, P.: Depth-dependent photoadaptation by zooxanthellae of the reef coral Montastrea annularis. Mar. Biol. 68, 253–264 (1982)Google Scholar
  8. Falkowski, P. G. and Z. Dubinsky: Light-shade adaptation of Stylophora pistillata, a hermatypic coral from the Gulf of Eilat. Nature, Lond. 289, 172–174 (1981)Google Scholar
  9. Fitt, W. K., S. S. Chang and R. K. Trench: Motility patterns of different strains of the symbiotic dinoflagellate Symbiodinium (=Gymnodinium) microadriaticum (Freudenthal) in culture. Bull. mar. Sci. 31, 436–443 (1981)Google Scholar
  10. Fitt, W. K., R. L. Pardy and M. M. Littler: Photosynthesis, respiration, and contribution to community productivity of the symbiotic sea anemone Anthopleura elegantissima (Brandt, 1835). J. exp. mar. Biol. Ecol. 61, 213–232 (1982)Google Scholar
  11. Franzisket, L.: The ratio of photosynthesis to respiration of reef building corals during a 24 hour period. Forma Functio 1, 153–158 (1969)Google Scholar
  12. Halldal, P.: Photosynthetic capacities and photosynthetic action spectra of endozoic algae of the massive coral Favia. Biol. Bull. mar. biol. Lab., Woods Hole 134, 411–424 (1968)Google Scholar
  13. Harding, L. W., Jr., B. B. Prézelin, B. M. Sweeney and J. L. Cox: Primary production as influenced by diel periodicity of phytoplankton photosynthesis. Mar. Biol. 67, 179–186 (1982)Google Scholar
  14. Jassby, A. D. and T. Platt. Mathematical formulation of the relationship between photosynthesis and light for phytoplankton. Limnol. Oceanogr. 21, 540–547 (1976)Google Scholar
  15. Jeffrey, S. W. and G. F. Humphrey. New spectrophotometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pfl. 167, 191–194 (1975)Google Scholar
  16. Jokiel, P. L. and R. H. York, Jr.: Solar ultraviolet photobiology of the reef coral Pocillopora damicornis and symbiotic zooxanthellae. Bull. mar. Sci. 32, 301–315 (1982)Google Scholar
  17. Lowry, C. H., N. J. Rosebrough, A. L. Farr and R. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)Google Scholar
  18. Marqhardt, D. W.: An algorithm for least-squares estimation of nonlinear parameters. J. Soc. ind. appl. Math. 11, 431–441 (1963)Google Scholar
  19. McCloskey, L. R. and L. Muscatine: Production and respiration in the Red Sea coral Stylophora pistillata as a function of depth. Proc. R. Soc. (Ser. B) (in press) (1984)Google Scholar
  20. Mergner, H. and A. Svoboda: Productivity and seasonal changes in selected reef. areas in the Gulf of Aqaba (Red Sea). Helgoländer wiss. Meeresunters. 30, 383–399 (1977)Google Scholar
  21. Muscatine, L.: Productivity of zooxanthellae. In: Primary productivity in the sea, pp 381–402 Ed. by P. G. Falkowski. New York: Plenum Press 1980Google Scholar
  22. Muscatine, L., L. R. McCloskey and R. E. Marian: Estimating the daily contribution of carbon from zooxanthellae to coral animal respiration. Limnol. Oceanogr. 26, 601–611 (1981)Google Scholar
  23. Parsons, T. R., M. Takahashi and B. Hargrave: Biological oceanographic processes, Oxford: Pergamon Press 1977Google Scholar
  24. Prézelin, B. B., B. W. Meeson and B. M. Sweeney: Characterization of photosynthetic rhythms in marine dinoflagellates. I. Pigmentation, photosynthetic capacity and respiration. Pl. Physiol., Baltimore, Md. 60, 384–387 (1977)Google Scholar
  25. Prézelin, B. B. and B. M. Sweeney: Characterization of photosynthetic rhythms in marine dinoflagellates. II. Photosynthesis-irradiance curves and in vivo chlorophyll a fluorescence. Plant Physiol., Baltimore, Md. 60, 388–392 (1977)Google Scholar
  26. Scott, B. D. and H. R. Jitts: Photosynthesis of phytoplankton and zooxanthellae on a coral reef. Mar. Biol. 41, 307–315 (1977)Google Scholar
  27. Shibata, K.: Pigments and a UV-absorbing substance in corals and a blue-green alga living in the Great Barrier Reef. Pl. Cell Physiol., Tokyo 10, 325–335 (1969)Google Scholar
  28. Svoboda, A.: In situ monitoring of oxygen production and respiration in Cnidaria with and without zooxanthellae. In: Physiology and behaviour of marine organisms, pp 75–82. Ed. by D. S. McLusky and A. J. Berry. Oxford: Pergamon Press 1978Google Scholar
  29. Trench, R. K. and C. R. Fisher: Carbon dioxide fixation in Symbiodinium microadriaticum: problems with mechanisms and pathways. In: Endocytobiology, pp 659–673. Ed. by H. E. A. Schenk and W. Schwemmler. Berlin: Walter de Gruyter 1983Google Scholar
  30. Trench, R. K., D. S. Wethey and J. W. Porter: Observations on the symbiosis with zooxanthellae among the Tridacnidae (Mollusca, Bivalvia). Biol. Bull. mar. biol. Lab., Woods Hole 161, 180–198 (1981)Google Scholar
  31. Wethey, D. S. and J. W. Porter: Sun and shade differences in productivity of reef corals. Nature, Lond. 262, 281–282 (1976)Google Scholar
  32. Wilkerson, F. P., G. Muller-Parker and L. Muscatine: Temporal patterns of cell division in natural populations of endosymbiotic algae. Limnol. Oceanogr. 28, 1009–1014 (1983)Google Scholar
  33. Zvalinskii, V. I., V. A. Leletkin, E. A. Titlyanov and M. G. Shaposhnikova: Photosynthesis and adaptation of corals to irradiance. 2. Oxygen exchange. Photosynthetica (Praha, Czechoslovakia) 14, 422–430 (1980)Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • G. Muller-Parker
    • 1
  1. 1.Department of BiologyUniversity of California at Los AngelesLos AngelesUSA

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