Advertisement

Planta

, Volume 80, Issue 3, pp 309–316 | Cite as

Untersuchungen über die lichtabhängige Carotinoidsynthese

III. Die farbstoffbildung von Neurospora crassa in submerskultur
  • W. Rau
  • I. Lindemann
  • A. Rau-Hund
Article

Light-dependent carotenoid synthesis

III. Pigment production of Neurospora crassa in submerged culture

Summary

In conidia-free submerged cultures of Neurospora crassa the various steps of light-dependend carotenoid synthesis were studied. The mycelium produces small amounts of pigments even in the dark. The data obtained are in part in good agreement with earlier results of Zalokar and show that the light-induced pigment production starts after a lag-period of 40 min and is finished after 6–8 hours; the photoreaction is saturated by relatively small dosages. In contrast to Zalokar's results we found that for photoinduction the reciprocity law holds true. The photoreaction is saturated by a certain amount of light independently of the light-intensity. Actidion (Cycloheximide) inhibits carotenoid synthesis completely when added before or up to 10 min after the onset of illumination, whereas addition 60 min after illumination already has no effect. Comparison with the results obtained with Fusarium shows that the reaction mechanism is very similar in both organisms, though the various steps seem to proceed faster in Neurospora.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Bradford, S. W., and B. J. Gibgot: Rapid production of Neurospora hyphae. Neurospora Newsletters 4, 17–19 (1963).Google Scholar
  2. Eberhard, D., W. Rau u. C. Zehender: Über den Einfluß des Lichts auf die Carotinoidbildung von Fusarium aquaeductuum. Planta (Berl.) 56, 302–308 (1961).Google Scholar
  3. Frost, L. C.: Conidiating media for Neurospora. Neurospora Newsletters 1, 11 (1962).Google Scholar
  4. Kandeler, R.: Über die Lichtabhängigkeit der Anthocyanbildung. Flora 149, 487–519 (1960).Google Scholar
  5. Krzeminsky, L. F., and F. W. Quackenbush: Stimulation of carotene synthesis in submerged cultures of Neurospora crassa by surface-active agents and ammonium nitrate. Arch. Biochem. 88, 64–67 (1960).Google Scholar
  6. Rau, W.: Untersuchungen über die lichtabhängige Carotinoidsynthese. I. Das Wirkungsspektrum von Fusarium aquaeductuum. Planta (Berl.) 72, 14–28 (1967a).Google Scholar
  7. —: Untersuchungen über die lichtabhängige Carotinoidsynthese. II. Ersatz der Lichtinduktion durch Mercuribenzoat. Planta (Berl.) 74, 263–277 (1967b).Google Scholar
  8. Rilling, H. C.: Photoinduction of carotenoid synthesis of a Mycobacterium sp. Biochim. biophys. Acta (Amst.) 60, 548–556 (1962).Google Scholar
  9. —: On the mechanism of photoinduction of carotenoid synthesis. Biochim. biophys. Acta (Amst.) 79, 464–475 (1964).Google Scholar
  10. Thomas, D. M., R. C. Harris, J. T. O. Kirk, and T. W. Goodwin: Studies on carotenogenesis in Blakeslea trispora. II. The mode of action of trisporic acid. Phytochemistry 6, 361–366 (1967).Google Scholar
  11. Turian, G.: Synthetic conidiogenous media for Neurospora crassa. Nature (Lond.) 202, 1240 (1964).Google Scholar
  12. Wagner, E., J. Bienger u. H. Mohr: Die Steigerung der durch Phytochrom bewirkten Anthocyansynthese des Senfkeimlings (Sinapis alba L.) durch Chloramphenicol. Planta (Berl.) 75, 1–9 (1967).Google Scholar
  13. Zalokar, M.: Studies on biosynthesis of carotenoids in Neurospora crassa. Arch. Biochem. 50, 71–80 (1954).Google Scholar
  14. —: Biosynthesis of carotenoids in Neurospora. Action spectrum of photoactivation. Arch. Biochem. 56, 318–325 (1955).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • W. Rau
    • 1
  • I. Lindemann
    • 1
  • A. Rau-Hund
    • 1
  1. 1.Botanisches Institut der Universität MünchenMünchenDeutschland

Personalised recommendations