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Planta

, Volume 168, Issue 3, pp 330–336 | Cite as

Changes in topography and function of thylakoid membranes following membrane protein phosphorylation

  • M. T. Black
  • P. Lee
  • P. Horton
Article

Abstract

Changes in topography and function of pea (Pisum sativum L.) thylakoid membrane fractions following membrane protein phosphorylation have been studied. After protein phosphorylation the stromal membrane fraction had a higher chlorophyll a/b ratio, an increased content of light-harvesting chlorophyll protein and a higher ratio of chlorophyll to cytochrome f. This indicates that a pool of light-harvesting chlorophyll protein migrates from the photosystem II-enriched grana regions to the photosystem I-enriched stroma lamellae, in agreement with Kyle et al. (1984, Biochim. Biophys. Acta 765, 89–96) and Larsson et al. (1983, Eur. J. Biochem. 136, 25–29). Phosphorylation caused a stimulation in the rate of light-limited photosystem-I electron transfer in the unappressed membrane fraction, indicating that the translocated LHC-II becomes functionally associated with photosystem I.

Key words

Chlorophyll protein complex Photosystem I, II Pisum (thylakoid) Protein kinase Thylakoid fractionation 

Abbreviations

Fm

fluorescence yield when all traps are closed

Fo

fluorescence yield when all traps are open

Fv

Fm-Fo

LHC-II

light-harvesting chlorophyll protein associated with photosystem II

PSI, II

photosystem I, II

P700

primary donor of PSI

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • M. T. Black
    • 1
    • 2
  • P. Lee
    • 1
    • 2
  • P. Horton
    • 1
    • 2
  1. 1.Research Institute for PhotosynthesisUniversity of SheffieldSheffieldUK
  2. 2.Department of BiochemistryUniversity of SheffieldSheffieldUK

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