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Changes in topography and function of thylakoid membranes following membrane protein phosphorylation


Changes in topography and function of pea (Pisum sativum L.) thylakoid membrane fractions following membrane protein phosphorylation have been studied. After protein phosphorylation the stromal membrane fraction had a higher chlorophyll a/b ratio, an increased content of light-harvesting chlorophyll protein and a higher ratio of chlorophyll to cytochrome f. This indicates that a pool of light-harvesting chlorophyll protein migrates from the photosystem II-enriched grana regions to the photosystem I-enriched stroma lamellae, in agreement with Kyle et al. (1984, Biochim. Biophys. Acta 765, 89–96) and Larsson et al. (1983, Eur. J. Biochem. 136, 25–29). Phosphorylation caused a stimulation in the rate of light-limited photosystem-I electron transfer in the unappressed membrane fraction, indicating that the translocated LHC-II becomes functionally associated with photosystem I.

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fluorescence yield when all traps are closed


fluorescence yield when all traps are open




light-harvesting chlorophyll protein associated with photosystem II


photosystem I, II


primary donor of PSI


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Black, M.T., Lee, P. & Horton, P. Changes in topography and function of thylakoid membranes following membrane protein phosphorylation. Planta 168, 330–336 (1986). https://doi.org/10.1007/BF00392357

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Key words

  • Chlorophyll protein complex
  • Photosystem I, II
  • Pisum (thylakoid)
  • Protein kinase
  • Thylakoid fractionation