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Planta

, Volume 171, Issue 4, pp 565–568 | Cite as

Spinach ferredoxin is a calcium-binding protein

  • Barbara Surek
  • Georg Kreimer
  • Michael Melkonian
  • Erwin Latzko
Short Communication

Abstract

Spinach-leaf ferredoxin was identified as a calcium-binding protein by 45Ca autoradiography on nitrocellulose membranes and with the cationic carbocyanine dye 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl] naphtho[1,2-d]thiazolium bromide (“stains-all”). Binding of 45Ca was observed at pH 6.8 and pH 7.8 and in the presence of 5 mM and 20 mM MgCl2. At the higher MgCl2 concentration the Ca2+-binding capacity is reduced. Only micromolar concentrations of LaCl3, however, are required to achieve a similar effect. Both the oxidized and reduced forms of ferredoxin bind calcium.

Key words

Calcium binding Ferredoxin (Ca2+-binding) Spinacia (Ca2+ binding) “Stains-all” (Ca2+ binding) 

Abbreviations

PAGE

polyacrylamide gel electrophoresis

SDS

sodium dodecyl sulfate

“stains-all”

1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl] naptho[1,2-d]thiazolium bromide

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Barbara Surek
    • 1
  • Georg Kreimer
    • 1
  • Michael Melkonian
    • 1
  • Erwin Latzko
    • 1
  1. 1.Botanisches Institut der Westfälischen Wilhelms-UniversitätMünsterFederal Republic of Germany

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