, Volume 165, Issue 3, pp 354–358 | Cite as

Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine

  • R. Reski
  • W. O. Abel


The bud-inducing effect of the cytokinin N6-(Δ2-isopentenyl)-adenine (i6-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i6-Ade, bud-formation was accelerated in both types of tissue. The number of buds, their size and their site of development were dependent on the concentration of the cytokinin in the range of 10-7 M to 10-5 M. Moreover, the percentage of caulonema cells increased with a cytokinin concentration of 10-5 M. These results indicate that chloronema cells may also function as target cells for exogenous cytokinins. The composition of proteins from caulonemata and chloronemata of two different species (P. patens and Funaria hygrometrica), grown on solid medium were compared. No differences could be detected between the protein patterns of caulonemata and chloronemata of the same species while between the two species the differences were obvious.

Key words

Bryophyta Bud induction (mosses) Caulonema, specific proteins Cytokinin and bud induction (mosses) Differentiation (moss) Funaria Physcomitrella 







sodium dodecyl sulfate-polyacrylamide gel electrophoresis


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  1. Ashton, N.W., Cove, D.J. (1977) The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens. Mol. Gen. Genet. 154, 87–95Google Scholar
  2. Ashton, N.W., Cove, D.J., Featherstone, D.R. (1979a) The isolation and physiological analysis of mutants of the moss, Physcomitrella patens, which overproduce gametophores. Planta 144, 437–442Google Scholar
  3. Ashton, N.W., Grimsley, N.H., Cove, D.J. (1979b) Analysis of gametophytic development in the moss, Physcomitrella patens, using auxin and cytokinin resistant mutants. Planta 144, 427–435Google Scholar
  4. Beutelmann, P., Bauer, L. (1977) Purification and identification of a cytokinin from moss callus cells. Planta 133, 215–217Google Scholar
  5. Bopp, M. (1963) Development of the protonema and bud formation in mosses. J. Linn. Soc. London, Bot. 58, 305–309Google Scholar
  6. Bopp, M. (1974) Action mechanism of cytokinins in mosses (a model for the effects of cytokinins). In: Plant growth substances 1973 (Proc. 8th Int. Conf. Plant Growth Subst.) pp. 934–944. Hirokawa Publishing Co. TokyoGoogle Scholar
  7. Bopp, M. (1981) Entwicklungsphysiologie der Moose. In: Advances in bryology, vol. 1, pp. 11–77, Schultze-Motel, W., ed. Cramer, BraunschweigGoogle Scholar
  8. Bopp, M. (1982) How can external hormones regulate the morphogenesis of mosses? J. Hattori Bot. Lab. 53, 159–169Google Scholar
  9. Bopp, M., Brandes, H. (1964) Versuche zur Protonemaentwicklung der Laubmoose. Planta 62, 116–136Google Scholar
  10. Bopp, M., Jahn, H., Klein, B. (1964) Eine einfache Methode, das Substrat während der Entwicklung von Moosprotonemen zu wechseln. Rev. Bryol. Lichén. 83, 219–223Google Scholar
  11. Bradford, M.M. (1976) A rapid and sensitivie method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254Google Scholar
  12. Brandes, H. (1967) Die Wirkung von Kinetin auf die Entwicklung verschiedener Laubmoosarten. Rev. Bryol. Lichén. 35, 158–164Google Scholar
  13. Erichsen, J., Knoop, B., Bopp, M. (1977) On the action mechanism of cytokinins in mosses: caulonema specific proteins. Planta 135, 161–168Google Scholar
  14. Hahn, H., Bopp, M. (1968) A cytokinin test with high specifity. Planta 83, 115–118Google Scholar
  15. Iwasa, K. (1965) Chemical control of morphogenesis in moss protonema. Plant Cell Physiol. 6, 421–429Google Scholar
  16. Laemmli, U.K., Favre, M. (1973) Maturation of the head of bacteriophage T4. J. Mol. Biol. 80, 575–599Google Scholar
  17. Morrissey, J.H. (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117, 301–310Google Scholar
  18. Nehlsen, W. (1979) A new method for examining induction of moss buds by cytokinin. Am. J. Bot. 66, 601–603Google Scholar
  19. Sood, S., Brenner, K., Bopp, M. (1978) The occurrence of caulonema-specific proteins in different moss species. Planta 138, 299–301Google Scholar
  20. Sood, S., Chopra, R.N. (1973) A record preponement of bud-induction in the moss Entodon myurus. Z. Pflanzenphysiol. 69, 390–393Google Scholar
  21. Szweykowska, A. (1963) Kinetin-induced formation of gametophores in dark cultures of Ceratodon purpureus. J. Exp. Bot. 14, 137–141Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. Reski
    • 1
  • W. O. Abel
    • 1
  1. 1.Institut für Allgemeine BotanikHamburg 52Federal Republic of Germany

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