β-Glucan biosynthesis in synchronous cells of Prototheca zopfii
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Abstract
Synchronous cultures of the colorless chlorophyte Prototheca zopfii Kruger were obtained by a shift-down of the basal medium plus addition of 1.5 mM ethylenediaminetetracetic acid for a 10–14-h period, followed by periodic dilutions with the basal medium. The cell cycle has a duration of 10–12 h at 25°C and an average of eight autospores are produced at the end of each cycle. The incorporation of [14C]glucose into β-glucans was determined in vivo. Very high incorporation occurs between hours 3 and 4 of culture, coincident with maximal cell expansion. The incorporation of glucose from uridine 5′diphospho-[14C]glucose into lipid-linked sugars and glycoproteins, as well as the activity of guanosine 5′-diphosphoglucose:β-glucan synthase found in cell-free preparations are in good agreement with the in-vivo incorporation of glucose into β-glucans.
Key words
β-Glucan synthesis chlorophyta Prototheca Synchrony induction (Prototheca)Abbreviations
- Glc
glucose
- GDP-Glc
guanosine 5′-diphosphoglucose
- UDP-Glc
uridine 5′-diphosphoglucose
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