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Planta

, Volume 165, Issue 1, pp 59–67 | Cite as

Reactivity of glyoxylate with hydrogen perioxide and simulation of the glycolate pathway of C3 plants and Euglena

  • A. Yokota
  • S. Kitaoka
  • K. Miura
  • A. Wadano
Article

Abstract

The nonenzymatic reaction of glyoxylate and H2O2 was measured under physiological conditions of the pH and concentrations of reactants. The reaction of glyoxylate and H2O2 was secondorder, with a rate constant of 2.27 l mol-1 s-1 at pH 8.0 and 25° C. The rate constant increased by 4.4 times in the presence of Zn2+ and doubled at 35°C. We propose a mechanism for the reaction between glyoxylate and H2O2. From a comparison of the rates of H2O2 decomposition by catalase and the reaction with glyoxylate, we conclude that H2O2 produced during glycolate oxidation in peroxisomes is decomposed by catalase but not by the reaction with glyoxylate, and that photorespiratory CO2 originates from glycine, but not from glyoxylate, in C3 plants. Simulation using the above rate constant and reported kinetic parameters leads to the same conclusion, and also makes it clear that alanine is a satisfactory amino donor in the conversion of glyoxylate to glycine. Some serine might be decomposed to give glycine and methylene-tetrahydrofolate; the latter is ultimately oxidized to CO2. In the simulation of the glycolate pathway of Euglena, the rate constant was high enough to ensure the decarboxylation of glyoxylate by H2O2 to produce photorespiratory CO2 during the glycolate metabolism of this organism.

Key words

C3 plant Euglena Glycolate pathway (simulation) Glyoxylate 

Abbreviations

Chl

chlorophyll

GGT

glutamate: glyoxylate aminotransferase (EC 2.6.1.4)

Hepes

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

SGT

serine: glyoxylate aminotransferase (EC 2.6.1.45)

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • A. Yokota
    • 1
  • S. Kitaoka
    • 1
  • K. Miura
    • 1
  • A. Wadano
    • 1
  1. 1.Department of Agricultural ChemistryUniversity of Osaka PrefectureSakai, OsakaJapan

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