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Planta

, Volume 165, Issue 1, pp 37–42 | Cite as

Phosphorylation of chloroplast membrane proteins partially protects against photoinhibition

  • P. Horton
  • P. Lee
Article

Abstract

Thylakoids isolated from peas (Pisum sativum cv. Kelvedon Wonder) and phosphorylated by incubation with ATP have been compared with non-phosphorylated thylakoids in their sensitivity to photoinhibition by exposure to illumination in vitro. Assays of the kinetics of fluorescence induction at 20° C and the fluorescence emission spectra at-196° C indicate a proportionally larger decrease in fluorescence as a result of photoinhibitory treatment of non-phosphorylated compared with phosphorylated thylakoids. It is concluded that protein phosphorylation can afford partial protection to thylakoids exposed to photoinhibitory conditions.

Key words

Chlorophyll fluorescence Chloroplast membrane Photoinhibition of photosynthesis Photosynthesis (photoinhibition) Pisum (photosynthesis) Protein phosphorylation 

Abbreviations and symbols

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

F0

Level of chlorophyll fluorescence when photosystem 2 traps are open

Fm

Level of chlorphyll fluorescence when photosystem 2 traps are closed

P

Maximum level of fluorescence reached in the absence of DCMU

PSI (II)

photosystem I(II)

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • P. Horton
    • 1
    • 2
  • P. Lee
    • 1
    • 2
  1. 1.Research Institute for PhotosynthesisUniversity of SheffieldSheffieldUK
  2. 2.Department of BiochemistryUniversity of SheffieldSheffieldUK

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