Planta

, Volume 178, Issue 3, pp 342–352 | Cite as

Comparative biochemical and immunological studies of the glycine betaine synthesis pathway in diverse families of dicotyledons

  • Elizabeth A. Weretilnyk
  • Sebastian Bednarek
  • Kent F. McCue
  • David Rhodes
  • Andrew D. Hanson
Article

Abstract

Members of the Chenopodiaceae can accumulate high levels (>100 μmol·(g DW)-1) of glycine betaine (betaine) in leaves when salinized. Chenopodiaceae synthesize betaine by a two-step oxidation of choline (choline→betaine aldehyde→ betaine), with the second step catalyzed by betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8). High betaine levels have also been reported in leaves of species from several distantly-related families of dicotyledons, raising the question of whether the same betaine-synthesis pathway is used in all cases.

Fast atom bombardment mass spectrometry showed that betaine levels of >100 μmol·(g DW)-1 are present in Lycium ferocissimum Miers (Solanaceae), Helianthus annuus L. (Asteraceae), Convolvulus arvensis L. (Convolvulaceae), and Amaranthus caudatus L. (Amaranthaceae), that salinization promotes betaine accumulation in these plants, and that they can convert supplied choline to betaine aldehyde and betaine. Nicotiana tabacum L. and Lycopersicon lycopersicum (L.) Karst. ex Farw. (Solanaceae), Lactuca sativa L. (Asteraceae) and Ipomoea purpurea L. (Convolvulaceae) also contained betaine, but at a low level (0.1–0.5 μmol·(g DW)-1. Betaine aldehyde dehydrogenase activity assays, immunotitration and immunoblotting demonstrated that the betaine-accumulating species have a BADH enzyme recognized by antibodies raised against BADH from Spinacia oleracea L. (Chenopodiaceae), and that the Mr of the BADH monomer is in all cases close to 63 000. These data indicate that the choline→betaine aldehyde→betaine pathway may have evolved by vertical descent from an early angiosperm ancestor, and might be widespread (albeit not always strongly expressed) among flowering plants. Consistent with these suggestions, Magnolia x soulangiana was found to have a low level of betaine, and to express a protein of Mr 63 000 which cross-reacted with antibodies to BADH from Spinacia oleracea.

Key words

Glycine betaine pathway Betaine aldehyde dehydrogenase Dicotyledons (glycine betaine) Fast atom bombardment mass spectrometry Salt stress 

Abbreviations

BADH

Betaine aldehyde dehydrogenase

DCIMS

desorption chemical ionization mass spectrometry

FABMS

fast atom bombardment mass spectrometry

Mr

relative molecular mass

PAGE

polyacrylamide gel electrophoresis

SDS

sodium dodecyl sulfate

TLC

thin-layer chromatography

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Elizabeth A. Weretilnyk
    • 1
  • Sebastian Bednarek
    • 1
  • Kent F. McCue
    • 1
  • David Rhodes
    • 2
  • Andrew D. Hanson
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast LansingUSA
  2. 2.Department of HorticulturePurdue UniversityWest LafayetteUSA

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