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Development of the preprophase band from random cytoplasmic microtubules in guard mother cells of Allium cepa L.

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Abstract

The organization of microtubule (MT) arrays in the guard mother cells (GMCs) of A. cepa was examined, focussing on the stage at which a longitudinal preprophase band (PPB) is established perpendicular to all other division planes in the epidermis. In the majority of young GMCs, including those seen just after asymmetric division, MTs are distributed randomly throughout the cortex and inner regions of the cytoplasm. Few MTs are associated with the nuclear surface. As the GMCs continue to develop, MTs cluster around the nucleus and a PPB appears as a wide longitudinal band. Microtubules also become prominent between the nucleus and the periclinal and transverse walls, while they decrease in number along the radial longitudinal walls. The PPB progressively narrows by early prophase, and a transversely oriented spindle gradually ensheaths the nucleus. These observations indicate that the initial, broad PPB is organized by a rearrangement of the random cytoplasmic array of MTs. Additional reorganization is responsible for MTs linking the nucleus and the cortex in the future plane of the cell plate, and for narrowing of the PPB.

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Abbreviations

GMC:

guard mother cell

MT:

microtubule

PPB:

preprophase band

References

  1. Gunning, B.E.S. (1982) The cytokinetic apparatus: its development and spatial regulation. In: The cytoskeleton in plant growth and development, pp. 229–292, Lloyd, C.W., (ed.). Academic Press, London

  2. Gunning, B.E.S., Hardham, A.R., Hughes, J.E. (1978) Preprophase bands of microtubules in all categories of formative and proliferative cell division in Azolla roots. Planta 143, 145–160

  3. Mabuchi, I., Tsukita, S., Tsukita, S., Sawai, T. (1988) Cleavage furrow isolated from newt eggs: Contraction, organization of the actin filaments, and protein components of the furrow. Proc. Natl. Acad. Sci. USA 85, 5966–5970

  4. Marc, J., Hackett, W.P. (1989) A new method for immunolocalization of microtubules in surface cell layers: application to the shoot apical meristem of Hedera. Protoplasma (148, 70–79)

  5. Mineyuki, Y., Marc, J., Palevitz, B.A. (1988a) Formation of the oblique spindle in dividing guard mother cells of Allium. Protoplasma 147, 200–203

  6. Mineyuki, Y., Wick, S.M., Gunning, B.E.S. (1988b) Preprophase bands of microtubules and the cell cycle: Kinetics and experimental uncoupling of their formation from the nuclear cycle in onion root-tip cells. Planta 174, 518–526

  7. Mullinax, J.B., Palevitz, B.A. (1989) Microtubule reorganization accompanying preprophase band formation in guard mother cells of Avena sativa L. Protoplasma (in press)

  8. Palevitz, B.A., Hepler, P.K. (1974) The control of the plane of division during stomatal differentiation in Allium. I. Spindle reorientation. Chromosoma 46, 297–326

  9. Wada, M., Mineyuki, Y., Kadota, A., Furuya, M. (1980) The changes of nuclear position and distribution of circumferentially aligned cortical microtubules during the progression of cell cycle in Adiantum protonemata. Bot. Mag. (Tokyo) 93, 237–245

  10. Wick, S.M., Duniec, J. (1983) Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Preprophase band development and concomitant appearance of nuclear envelope-associated tubulin. J. Cell Biol. 97, 235–243

  11. Wick, S.M., Seagull, R.W., Osborn, M., Weber, K., Gunning, B.E.S. (1981) Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J. Cell Biol. 89, 685–690

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Correspondence to B. A. Palevitz.

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Mineyuki, Y., Marc, J. & Palevitz, B.A. Development of the preprophase band from random cytoplasmic microtubules in guard mother cells of Allium cepa L.. Planta 178, 291–296 (1989). https://doi.org/10.1007/BF00391856

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Key words

  • Allium
  • Guard mother cell
  • Microtubule
  • Polarity
  • Preprophase band
  • Stomatal complex