Comparative studies of tumor-initiating activity in mouse skin and carcinogenicity in rat mammary gland were conducted with several dibenzo[a]-pyrenes (DBPs). SENCAR mice were initiated with DB[a,e]P, DB[a,h]P, DB[a,i]P, DB[a,l]P and anthanthrene, and promoted with tetradecanoyl-phorbol acetate. The same compounds were tested by intramammillary injection in female Sprague-Dawley rats. Anthanthrene was inactive in both mouse skin and rat mammary gland. DB[a,e]P was a very weak tumor-initiator in mouse skin and was inactive in rat mammary gland. DB[a,h]P induced twice as many papillomas in mouse skin as DB[a,i]P, although both compounds exhibited similar tumor latencies and percentages of tumor-bearing mice. These two compounds induced similar numbers of mammary tumors, but treatment of the rats with DB[a,i]P resulted in a significantly larger number of adenocarcinomas. DB[a,l]P was toxic to both the mice and rats. Treatment of mouse skin with this compound led to an erythema, which delayed the beginning of promotion until the 3rd week after initiation. Despite this delay, papillomas began appearing 5 weeks after initiation with DB[a,l]P and the number of tumors increased rapidly. The compound was so toxic in the rats that half of the animals died in the first 9 weeks and the remaining animals were sacrificed after 15 weeks. Nonetheless, DB[a,l]P was the strongest carcinogen tested, inducing seven tumors per rat within 10 weeks. These results demonstrate that DB[a,l]P, which is present in tobacco smoke, is an extremely potent carcinogenic aromatic hydrocarbon. Furthermore, some of these compounds can serve as useful models for elucidating their mechanisms of activation.
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This research was supported by Public Health Service grants CA32376 and CA25176 from the National Cancer Institute and by institutional grants CA36727 from the National Cancer Institute and ACS SIG-16 from the American Cancer Society
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Cavalieri, E.L., Rogan, E.G., Higginbotham, S. et al. Tumor-initiating activity in mouse skin and carcinogenicity in rat mammary gland of dibenzo[a]pyrenes: the very potent environmental carcinogen dibenzo[a, l]pyrene. J Cancer Res Clin Oncol 115, 67–72 (1989). https://doi.org/10.1007/BF00391602
- Mouse skin
- Rat mammary gland