Current Genetics

, Volume 16, Issue 3, pp 211–218 | Cite as

Dispersed repetitive sequences in the chloroplast genome of Douglas-fir

  • Ching-Hsiu Tsai
  • Steven H. Strauss
Original Articles


Restriction mapping and DNA sequencing were used to characterize dispersed repetitive DNA in the chloroplast genome of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. To map repeat families, chloroplast DNA (cpDNA) clones were hybridized at high stringency to one another and to cpDNA cut with restriction enzymes. Repeats are clustered in four regions of the genome and comprise at least six families. Sequence analysis of one repeat family shared among three XbaI fragments indicated the presence of a 633 by inverted repeat which contains a complete tRNA-Serine (GCU) gene and a highly conserved open reading frame (ORF 3.6). Both ends of this 633 bp dispersed repeat have a transposon-like combination of short direct and inverted repeats. One copy of the repeat flanks one of the endpoints of a major inversion which differentiates Douglas-fir from tobacco cpDNA. Dispersion of repetitive DNA by transposition, coupled with loss of the large inverted repeat, appears to have predisposed conifer cpDNA to a number of inversions. An 8 by (CATCTTTT) direct repeat in tobacco is located between two inverted sections in Douglas-fir; it may be a target sequence for homologous recombination.

Key words

Chloroplast DNA Repetitive DNA Inversion Recombination Transposition 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Ching-Hsiu Tsai
    • 1
  • Steven H. Strauss
    • 1
  1. 1.Department of Forest Science, Peavy Hall 154Oregon State UniversityCorvallisUSA

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