Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The genes of both subunits of ribulose-1,5-bisphosphate carboxylase constitute an operon on the plastome of a red alga

  • 54 Accesses

  • 77 Citations

Summary

Plastid (pt) DNA from the red alga Porphyridium aerugineum was purified by CsCI gradient centrifugation. An EcoRI library of the ptDNA was screened with a gene probe specific for the gene encoding the large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco EC 4.1.1.39) from spinach. A 5.8 kb EcoRI clone containing the LSU gene (rbcL) was isolated and the DNA sequence of the Porphyridium rbcL gene and its flanking regions was determined. An open reading frame was found 130 by downstream from the rbcL gene that shows homology to genes coding for the small subunit of Rubisco (rbcS) from higher plants and cyanobacteria. Both genes (rbcL + rbcS) are cotranscribed. Comparison of rbcL and rbcS sequences from Porphyridium, higher plants and cyanobacteria seems to reveal a remarkable evolutionary distance between the plastids of the red algae (rhodoplasts), chloroplasts and cyanobacteria.

This is a preview of subscription content, log in to check access.

References

  1. Bedbrook JR, Smith SM, Ellis RJ (1980) Nature 287:692–697

  2. Curtis SE, Haselkorn R (1983) Proc Natl Acad Sci USA 80:1835–1839

  3. Davies LG, Dibner MD, Battey JF (1986) Basic methods in molecular biology. Elsevier Scientific Publ, Amsterdam

  4. Hunt IT, George DG, Barker WA (1985) BioSystems 18:223–240

  5. Kaling M (1983) Inaugural Dissertation. Justus Liebig Universität Giessen, FRG

  6. Kröger M, Kröger-Block A (1984) Nucleic Acids Res 12:113–120

  7. Kuhsel M, Kowallik KV (1987) Mol Gen Genet 207:361–368

  8. Li N, Cattolico RA (1987) Mol Gen Genet 209:343–247

  9. Linne v Berg K-H, Kowallik KV (1988) BioSystems 21:239–247

  10. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

  11. Margulis L (1981) Symbiosis in cell evolution, W.H. Freeman & Co, San Francisco

  12. Newsman S, Cattolico RA (1988) Plant Mol Biol 11:821–831

  13. Nierzwicki-Bauer SA, Curtis SE, Haselkorn R (1984) Proc Natl Acad Sci USA 81:5961–5965

  14. Ohyama K, Fukuda H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H (1986) Nature 322:572–574

  15. Palmer JD (1985) Annu Rev Genet 19:325–345

  16. Perasso R, Baroin A, Qu LH, Bachellerie JP, Adoutte A (1989) Nature 339:142–144

  17. Reith M, Cattolico RA (1986) Proc Natl Acad Sci USA 83:8599–8603

  18. Ruf M, Kössel H (1988) FEBS Lett 240:41–44

  19. Sanger F, Nicklen S, Coulsen AR (1977) Proc Natl Acad Sci USA 74:5463–5468

  20. Schlösser UG (1982) Ber Dtsch Bot Ges 95:181–276

  21. Shine J, Dalgarno L (1975) Nature 254:34–38

  22. Shinozaki K, Sugiura M (1983) Nucleic Acids Res 11:6957–6964

  23. Shinozaki K, Sugiura M (1985) Mol Gen Genet 200:27–32

  24. Shinozaki K, Yamada C, Takahata N, Sugiura M (1983) Proc Natl Acad Sci USA 80:4050–4054

  25. Shinozaki K, Ohme M, Tanaka M, Wakasuki T, Hayashida N, Matsubayashi T, Zaita T, Chungwongse J, Obokata J, Yamagushi-Shinozaki K, Ohto C, Torezawa K, Meng B, Sugita Y, Deno H, Kamogashira T, Yamada K, Kusuda J, Taikawa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049

  26. Starnes SM, Lambert DH, Maxwell ES, Stevens SE jr, Porter RD, Shively JM (1985) FEBS Lett 28:165–169

  27. Steinmüller K, Kaling M, Zetsche K (1983) Planta 159:308–313

  28. Sugita M, Manzara T, Pichersky E, Cashmore A, Gruissem W (1987) Mol Gen Genet 209:247–256

  29. Zurawski G, Perrot B, Bottomly W, Whitfield PR (1981) Nucleic Acids Res 9:3251–3269

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Valentin, K., Zetsche, K. The genes of both subunits of ribulose-1,5-bisphosphate carboxylase constitute an operon on the plastome of a red alga. Curr Genet 16, 203–209 (1989). https://doi.org/10.1007/BF00391478

Download citation

Key words

  • Red algae
  • ptDNA
  • Ribulose-1,5-bisphosphate carboxylase
  • Plastid evolution