The effect of indole-3-acetic acid on coleoptile extension growth in the absence of protein synthesis
Article
Received:
- 21 Downloads
- 13 Citations
Summary
In wheat coleoptile sections cycloheximide inhibited over 90% of 14C-leucine incorporation into protein within 10 minutes of its application. Even after 2-hour pretreatments with cycloheximide, IAA stimulated extension, suggesting that its growth-promoting action did not directly involve protein synthesis.
Kinetic experiments with cycloheximide indicate that incorporation of a structural factor (possibly protein from a previously synthesised pool) into cell walls might be the rate-limiting process affected by IAA.
Keywords
Cell Wall Protein Synthesis Structural Factor Cycloheximide Kinetic Experiment
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Preview
Unable to display preview. Download preview PDF.
References
- Burström, H. G., Uhrström, I., Olausson, B.: Influence of auxin on Young's modulus in stems and roots of Pisum and the theory of changing the modulus in tissues. Physiol. Plantarum (Cph.) 23, 1223–1233 (1970).Google Scholar
- Cleland, R.: Instability of the growth-limiting proteins of the Avena coleoptile and their pool size in relation to auxin. Planta (Berl.) 99, 1–11 (1971).Google Scholar
- Coartney, J. S., Morré, D. J., Key, J. L.: Inhibition of RNA synthesis and auxin-induced cell wall extensibility by actinomycin D. Plant Physiol. 42 434–439 (1967).Google Scholar
- De la Fuente, R., Leopold, A. C.: Time course of auxin-stimulation of growth. Plant Physiol. 46, 186–189 (1970).Google Scholar
- Ellis, R. J., MacDonald, I. R.: Specificity of cycloheximide in higher plant systems. Plant Physiol. 46, 227–232 (1970).Google Scholar
- Evans, M., Ray, P.: Timing of the auxin response in coleoptiles and its implications regarding auxin action. J. gen. Physiol. 53, 1–20 (1969).Google Scholar
- Jamieson, J. D., Palade, G. E.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. III. Dissociation of intracellular transport from protein synthesis. J. Cell Biol. 39, 580–588 (1968).Google Scholar
- Key, J. L.: Hormones and nucleic acid metabolism. Ann. Rev. Plant Physiol. 20, 449–474 (1969).Google Scholar
- Lamport, D.T.A.: The protein component of primary cell walls. In: R. D. Preston (ed.). Advanc. Botan. Res. 2, 151–218 (1965).Google Scholar
- Masuda, Y., Tanimoto, E., Wada, S.: Auxin-stimulated RNA synthesis in oat coleoptile cells. Physiol. Plantarum (Cph.) 20, 713–719 (1967).Google Scholar
- Morimoto, T., Tashiro, Y., Matsuura, S.: Chase of newly synthesized proteins in guinea-pig pancreas with cycloheximide. Biochim. biophys. Acta (Amst.) 138, 631–633 (1967).Google Scholar
- Nelson, H., Ilan, I., Reinhold, L.: The effect of inhibitors of protein and RNA synthesis on auxin-induced growth. Isrel J. Bot. 18, 129–134 (1969).Google Scholar
- Nissl, D., Zenk, M.: Evidence against induction of protein synthesis during auxin-induced initial elongation of Avena coleoptiles. Planta (Berl.) 89, 323–341 (1969).Google Scholar
- Noodén, L. D., Thimann, K. V.: Evidence for a requirement for protein synthesis for auxin-induced cell enlargement. Proc. nat. Acad. Sci. (Wash.) 50, 194–200 (1963).Google Scholar
- ——: Action of inhibitors of RNA and protein synthesis on cell enlargement. Plant Physiol. 41, 157–164 (1966).Google Scholar
- Ray, P.: The action of auxin on cell enlargement in plants. In: Lang (ed.), p. 172–199. Communication in development. New York and London. Academic Press 1969.Google Scholar
- Ray, P., Ruesink, A.: Kinetic experiments on the nature of the growth mechanism in oat coleoptile cells. Develop. Biol. 4, 377–397 (1962).Google Scholar
- Rayle, D. L., Cleland, R.: Enhancement of wall loosening and elongation by acid solutions. Plant Physiol. 46, 250–253 (1970).Google Scholar
- —, Ouitrakul, R., Hertel, R.: Effects of auxins on the auxin transport system in coleoptiles. Planta (Berl.) 87, 49–53 (1969).Google Scholar
- Thompson, E. W., Preston, R. D.: Evidence for a structural role of protein in algal cell walls. J. exp. Bot. 19, 690–697 (1968).Google Scholar
- Trewavas, A. J.: Relationship between plant growth and nucleic acid metabolism. In: Progress in phytochemistry (L. Reinhold and Y. Liwschitz, eds.), vol. 1, p. 113–160. New York: Interscience 1968.Google Scholar
Copyright information
© Springer-Verlag 1972