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The pyridine-nucleotide cycle in tobacco

Enzyme activities for the recycling of NAD

Abstract

In order to elucidate the NAD-recycling pathway the following enzyme activities have been characterized in different tobacco tissues and in tomato root: NAD pyrophosphatase, nicotinamide mononucleotide (NMN)/nicotinic acid mononucleotide (NaMN) glycohydrolases, nicotinamidase and nicotinic acid phosphoribosyltransferase. The investigations were performed with protein extracts purified by gel filtration and enzymatic activities were determined by high-performance liquid chromatography methods. The kinetic parameters of the different enzymes from tobacco root and their specificity are reported. The data are in favor of the so-called pyridine-nucleotide cycle VI (NAD→NMN→nicotinamide→nicotinic acid→NaMN→nicotinic acid adenine dinucleotide→NAD). In the nicotine-producing tobacco root a further direct route leading from NaMN to nicotinic acid is proposed. These data are reconciled with the assumption that it is nicotinic acid which is provided by the pyridine-nucleotide cycle for the synthesis of nicotine.

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Abbreviations

HPLC:

high-performance liquid chromatography

Na:

nicotinic acid

NaAD:

nicotinic acid adenine dinucleotide

NaMN:

nicotinic acid mononucleotide

NMN:

nicotinamide mononucleotide

PRPP:

5-phosphoribosyl-1-pyrophosphate

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Author information

Correspondence to K. G. Wagner.

Additional information

This contribution is dedicated to Professor Augustin Betz on the occasion of his 65th birthday

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Wagner, R., Feth, F. & Wagner, K.G. The pyridine-nucleotide cycle in tobacco. Planta 167, 226–232 (1986). https://doi.org/10.1007/BF00391419

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Key words

  • NAD pyrophosphatase
  • Nicotinamidase
  • Nicotiana (pyridine-nucleotide cycle)
  • Nicotine biosynthesis
  • Pyridine nucleotide cycle