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Planta

, Volume 167, Issue 3, pp 376–381 | Cite as

The relationship between phosphate status and photosynthesis in leaves

Reversibility of the effects of phosphate deficiency on photosynthesis
  • K.-J. Dietz
  • C. Foyer
Article

Abstract

Spinach (Spinacia oleracea L.) and barley (Hordeum vulgare L.) were grown in hydroponic culture with varying levels of orthophosphate (Pi). When leaves were fed with 20 mmol·l−1 Pi at low CO2 concentrations, a temporary increase of CO2 uptake was observed in Pi-deficient leaves but not in those from plants grown at 1 mmol·l−1 Pi. At high concentrations of CO2 (at 21% or 2% O2) the Pi-induced stimulation of CO2 uptake was pronounced in the Pi-deficient leaves. The contents of phosphorylated metabolites in the leaves decreased as a result of Pi deficiency but were restored by Pi feeding. These results demonstrate that there is an appreciable capacity for rapid Pi uptake by leaf mesophyll cells and show that the effects of long-term phosphate deficiency on photosynthesis may be reversed (at least temporarily) within minutes by feeding with Pi.

Key words

Hordeum (phosphate, photosynthesis) Orthophosphate (deficiency, photosynthesis) Photosynthesis (phosphate status) Spinacia (phosphate, photosynthesis) 

Abbreviation

Pi

orthophosphate

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References

  1. Arnon, D.I. (1949) Copper enzyme in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15Google Scholar
  2. Bielski, R.L. (1973) Phosphate pools, phosphate transport, and phosphate availability. Annu. Rev. Plant Physiol. 24, 225–252Google Scholar
  3. Bottrill, D.E., Possingham, J.V., Kriedemann, P.E. (1970) The effect of nutrient deficiencies on photosynthesis and respiration in spinach. Plant Soil 32, 424–438Google Scholar
  4. Bouma, D. (1975) Effects of metabolic phosphorus compounds on rates of photosynthesis of detached phosphorus-deficient subterranean clover leaves. J. Exp. Bot. 26, 52–59Google Scholar
  5. Cockburn, W., Baldry, C.W., Walker, D.A. (1967a) Oxygen evolution by isolated chloroplasts with carbon dioxide as the hydrogen acceptor. A requirement for orthophosphate or pyrophosphate. Biochim. Biophys. Acta 131, 594–596Google Scholar
  6. Cockburn, W., Baldry, C.W., Walker, D.A. (1967b) Some effects of inorganic phosphate on O2 evolution by isolated chloroplasts. Biochim. Biophys. Acta 143, 614–624Google Scholar
  7. Dietz, K.-J., Heber, U. (1984) Rate limiting factors in leaf photosynthesis. I. Carbon fluxes in the calvin cycle. Biochim. Biophys. Acta 767, 432–443Google Scholar
  8. Edwards, G.E., Walker, D.A. (1983) C3, C4 Mechanisms: Cellular and environmental regulation of photosynthesis, pp. 181–183, Blackwell Scientific publications, Oxford, LondonGoogle Scholar
  9. Falkner, G., Horner, F., Simonis, W. (1980) The regulation of the energy dependent phosphate uptake by the blue green alga Anacystis nidulans. Planta 149, 138–143Google Scholar
  10. Furbank, R.T., Walker, D.A. (1985) Photosynthetic induction in C4 leaves: An investigation using infra-red gas analysis and chlorophyll a fluorescence. Planta 163, 75–83Google Scholar
  11. Harris, G.C., Cheesbrough, J.K., Walker, D.A. (1983) Measurement of CO2 and H2O vapour exchange in spinach leaf discs: Effect of orthophosphate. Plant Physiol. 71, 102–107Google Scholar
  12. Heber, U., Willenbrink, J. (1964) Sites of synthesis and transport of photosynthetic products within the leaf cell. Biochim. Biophys. Acta 82, 313–324Google Scholar
  13. Heldt, H.W., Chon, C.J., Maronde, D., Herold, A., Stankovic, Z.S., Walker, D.A., Kraminer, A., Kirk, M.R., Heber, U. (1977) The role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiol. 59, 1146–1155Google Scholar
  14. Herold, A., Walker, D.A. (1979) Transport across chloroplast envelopes — the role of phosphate. In: Membrane transport in biology, vol. 2, pp. 411–439, Giebish, G., Tosterson, D., Ussing, H., eds. Springer, New YorkGoogle Scholar
  15. Machler, F., Schnyder, H., Nosberger, J. (1984) Influence of inorganic phosphate on photosynthesis of wheat chloroplasts. I. Photosynthesis and assimilate export at 5° and 25°C. J. Exp. Bot. 35, 481–487Google Scholar
  16. Mengel, K. (1969) Phosphorus. In: Handbuch für Pflanzenernährung, pp. 379–394, Linser, H., ed. Springer, New YorkGoogle Scholar
  17. Michal, G., Beutler, H.-O. (1974) d-fructose-1,6-diphosphate, dihydroxyacetone phosphate and d-glyceraldehyde-3-phosphate. In: Methods of enzymatic analysis vol., pp. 1314–1319, Bergmeyer, H.U., ed. Academic Press, New York LondonGoogle Scholar
  18. Natr, L. (1970) Effect of mineral nutrient deficiencies on dry matter production, photosynthetic intensity and N, P, and K quantities in plants. Physiol. Vég. 8, 573–583Google Scholar
  19. Pirson, A., Tichy, C., Wilhelmi, G. (1952) Stoffwechsel und Mineralsalzernährung einzelliger Grimalgen. 1. Vergleichende Untersuchungen an Mangelkulturen von Ankistrodesmus. Planta 40, 199–253Google Scholar
  20. Racker, E. (1974) D-ribulose-1,5-diphosphate. In: Methods of enzymatic analysis, vol. 3, pp. 1362–1364, Bergmeyer, H.U., ed. Academic Press, New York LondonGoogle Scholar
  21. Rebeille, F., Bligny, R., Douce, R. (1982) Regulation of Pi uptake by Acer pseudoplatanus cells. Arch. Biochem. Biophys. 219, 371–378Google Scholar
  22. Rebeille, F., Bligny, R., Martin, J.-B., Douce, R. (1983) Relationship between the cytoplasm and the vacuole phosphate pool in Acer pseudoplatanus cells. Arch. Biochem. Biophys. 225, 143–148Google Scholar
  23. Taussky, H.H., Shorr, E. (1953) A microcolorimetric method for the determination of inorganic phosphorus. J. Biol. Chem. 202, 675–685Google Scholar
  24. Terry, N., Ulrich, A. (1973) Effects of phosphorus deficiency on the photosynthesis and respiration of leaves of sugar beet. Plant Physiol. 51, 43–47Google Scholar
  25. Ullrich-Eberius, C.I., Novacky, A., van Bel, A.J.E. (1984) Phosphate uptake in Lemna gibba GI: energetics and kinetics. Planta 161, 46–52Google Scholar
  26. Walker, D.A. (1980) Regulation of starch synthesis in leaves — the role of orthophosphate. In: Physiological aspects of crop productivity. Proc. 15th Colloq. Int. Potash Inst., Bern, pp. 195–207Google Scholar
  27. Woodrow, I.E., Ellis, J.R., Jellings, A., Foyer, C.H. (1984) Compartmentation and fluxes of inorganic phosphate in photosynthetic cells. Planta 161, 525–530Google Scholar
  28. Zalitis, J., Uram, M., Bowser, A.M., Feingold, D.S. (1972) UDP-glucose dehydrogenase from beef liver. Methods Enzymol. 28, 430–435Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • K.-J. Dietz
    • 1
  • C. Foyer
    • 2
  1. 1.Institut für BotanikUniversität Würzburg, Lehrstuhl Botanik 1WürzburgFederal Republic of Germany
  2. 2.Research Institute for Photosynthesis, Department of BotanyUniversity of SheffieldSheffieldUK

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