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Kinetics of intracellular redistribution of phytochrome in Avena coleoptiles after its photoconversion to the active, far-red-absorbing form

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Abstract

The kinetics of the intracellular redistribution of phytochrome (sequestering) in Avena sativa L. coleoptiles following a brief, saturating actinic pulse of red (R) light have been determined. Immunocytochemical labelling of phytochrome with monoclonal antibodies showed that at 22°C sequestering can occur within 1–2 s from the onset of R irradiation and is dependent upon the continued presence of the far-red-absorbing form of phytochrome (Pfr). The initial rate, but not the final extent, of sequestering is reduced by lowering the temperature of the tissue to 1°C. Sequestering at 22°C appears to involve two distinct stages: (1) a rapid association of Pfr with putative binding sites initiates the sequestered condition, following which (2) these sites of sequestered phytochrome appear to aggregate. Neither of these two processes was affected by the cytoskeletal inhibitors colchicine or cytochalasin B. Phytochrome sequestering therefore resembles R-light-induced phytochrome pelletability with respect to kinetics, temperature sensitivity, and dependence upon the continued presence of Pfr in the cell.

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Abbreviations

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

DIC:

differential interference contrast

FR:

far-red

Ig:

immunoglobulin

Pfr, Pr:

far-red-absorbing and red-absorbing form of phytochrome, respectively

R:

red

References

  1. Coleman, R.A., Pratt, L.H. (1974a) Electron microscopic localization of phytochrome in plants using an indirect antibodylabelling method. J. Histochem. Cytochem. 22, 1039–1047

  2. Coleman, R.A., Pratt, L.H. (1974b) Subcellular localization of the red-absorbing form of phytochrome by immunocyto-chemistry. Planta 121, 119–131

  3. Cordonnier, M.-M., Smith, C., Greppin, H., Pratt, L.H. (1983) Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta 158, 369–376

  4. Cordonnier, M.-M., Greppin, H., Pratt, L.H. (1985) Monoclonal antibodies with differing affinities to the red-absorbing and far-red-absorbing forms of phytochrome. Biochemistry 24, 3246–3253

  5. Epel, B.L., Butler, W.L., Pratt, L.H., Tokuyasu, K.T. (1980) Immunofluorescence localization studies of the Pr and Pfr forms of phytochrome in the coleoptile tips of oats, corn and wheat. In: Photoreceptors and plant development, pp. 121–133, De Greef, J., ed. Antwerpen University Press, Antwerpen

  6. Giloh, H., Sedat, J.W. (1982) Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science 217, 1252–1255

  7. Hendricks, S.B., Borthwick, H.A. (1967) The function of phytochrome in regulation of plant growth. Proc. Natl. Acad. Sci. USA 58, 2125–2130

  8. Hepler, P.K., Palevitz, B.A. (1974) Microtubules and microfilaments. Annu. Rev. Plant Physiol. 25, 309–362

  9. Mackenzie, J.M., Jr., Coleman, R.A., Briggs, W.R., Pratt, L.H. (1975) Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. Proc. Natl. Acad. Sci. USA 72, 799–803

  10. Marmé, D. (1977) Phytochrome: membranes as possible sites of primary action. Annu. Rev. Plant Physiol. 28, 173–198

  11. Mohr, H. (1966) Differential gene activation as a mode of action of phytochrome 730. Photochem. Photobiol. 5, 469–453

  12. Pratt, L.H. (1977) Progress toward an understanding of the molecular mode of action of phytochrome. In: Research in photobiology, pp. 511–519, Castellani, A., ed. Plenum Press, New York

  13. Pratt, L.H. (1978) Molecular properties of phytochrome. Photochem. Photobiol. 27, 81–105

  14. Pratt, L.H. (1979) Phytochrome: function and properties. In: Photochem. and Photobiol. Rev., vol. 4, pp. 59–124, Smith, K.C., ed. Plenum Press, New York

  15. Pratt, L.H. (1984) Phytochrome purification. In: Techniques in photomorphogenesis, pp. 175–200, Smith, H., Holmes, M.G., eds. Academic Press, London New York

  16. Pratt, L.H., Coleman, R.A. (1974) Phytochrome distribution in etiolated grass seedlings as assayed by an indirect antibody-labelling method. Am. J. Bot. 61, 195–202

  17. Pratt, L.H., Marmé, D. (1976) Red-light enhanced phytochrome pelletability: re-examination and further characterization. Plant Physiol. 58, 686–692

  18. Quail, P.H. (1983) Rapid action of phytochrome in photomorphogenesis. In: Encyclopedia of plant physiology, vol. 16A: Photomorphogenesis, pp. 178–212, Shropshire, W., Jr., Mohr, H., eds. Springer, Berlin

  19. Quail, P.H., Briggs, W.R. (1978) Irradiation-enhanced phytochrome pelletability. Requirement for phosphorylative energy in vivo. Plant Physiol. 62, 773–778

  20. Saunders, M.J., Cordonnier, M.-M., Palevitz, B.A., Pratt, L.H. (1983) Immunofluorescence visualization of phytochrome in Pisum sativum L. epicotyls using monoclonal antibodies. Planta 159, 545–553

  21. Smith, H. (1975) Phytochrome and photomorphogenesis. McGraw-Hill, London

  22. Tobin, E.M., Silverthorne, J. (1985) Light regulation of gene expression in higher plants. Annu. Rev. Plant Physiol. 36, 569–593

  23. Verbelen, J.-P., Pratt, L.H., Butler, W.L., Tokuyasu, K. (1982) Localization of phytochrome in oats by electron microscopy. Plant Physiol. 70, 867–871

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McCurdy, D.W., Pratt, L.H. Kinetics of intracellular redistribution of phytochrome in Avena coleoptiles after its photoconversion to the active, far-red-absorbing form. Planta 167, 330–336 (1986). https://doi.org/10.1007/BF00391335

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Key words

  • Avena (phytochrome)
  • Monoclonal antibodies (phytochrome)
  • Phytochrome (sequestering)