Planta

, Volume 167, Issue 4, pp 469–472 | Cite as

Prophase bands of microtubules occur in protoplast cultures of Vicia hajastana Grossh

  • D. H. Simmonds
Article
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Accepted:

Abstract

Circumnuclear bands of microtubules (MT) have been found in the prophase of mitoses in cultured protoplasts of Vicia hajastana. The timing of the appearance and disappearance of the prophase band of MT (PB) relative to the stage of mitosis was studied using simultaneous staining of MT by immunofluorescence and DNA by Hoechst 33258. These protoplasts regenerate into unorganized tissue. Pre-prophase bands of MT have previously been found only in highly organized tissues of higher plants. The role of PB in cell division is discussed.

Key words

Microtubule Prophase band Protoplast Vicia 

Abbreviations

MT

microtubule(s)

PB

prophase band(s)

FPB

pre-prophase band(s)

PNF

perinuclear fluorescence

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References

  1. Busby, C.H., Gunning, B. (1980) Observations on pre-prophase bands of microtubule uniseriate hairs, stomatal complexes of sugar cane and Cyperus root meristems. Eur. J. Cell Biol. 21, 214–223Google Scholar
  2. De Mey, J., Lambert, A.M., Bajer, A.S., Moeremans, M., De Brabander, M. (1982) Visualization of microtubules in interphase and mitotic plant cells of Haemanthus endosperm with the immuno-gold staining method. Proc. Natl. Acad. Sci. USA 79, 1898–1902Google Scholar
  3. Fowke, L.C., Bech-Hansen, C.W., Constabel, F., Gamborg, O.L. (1974) A comparative study on the ultrastructure of cultured cells and protoplasts of soybean during cell division. Protoplasma 81, 189–203Google Scholar
  4. Galatis, B. (1982) The organization of microtubules in guard cell mother cells of Zea mays. Can. J. Bot. 60, 1148–1166Google Scholar
  5. Galatis, B., Mitrakos, K. (1979) On the differential divisions and preprophase microtubule bands involved in the development of stomata of Vigna sinensis L. J. Cell Sci 37, 11–37Google Scholar
  6. Gunning, B.E.S. (1982) The cytokinetic apparatus, its development and spatial regulation. In: The cytoskeleton in plant growth and development, pp. 229–292, Lloyd, C.W., ed. Academic Press, London New YorkGoogle Scholar
  7. Gunning, B.E.S., Hardham, A.R. (1982) Microtubules. Annu. Rev. Plant Physiol. 33, 651–698Google Scholar
  8. Gunning, B.E.S., Hardham, A.R., Hughes, J.E. (1978) Pre-prophase bands of microtubules in all categories of formative and proliferative cell division in Azolla roots. Planta 143, 145–160Google Scholar
  9. Kao, K.N., Michayluk, M.R. (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126, 105–110Google Scholar
  10. Palevitz, B.A., Hepler, P.K. (1974) The control of the plane of division during stomatal differentiation in Allium. I. Spindle reorientation. Chromosoma (Berl.) 46, 297–326Google Scholar
  11. Pickett-Heaps, J.D. (1969) Preprophase microtubules and stomatal differentiation in Commelina cyanea. Aust. J. Biol. Sci 22, 375–391Google Scholar
  12. Pickett-Heaps, J.D., Northcote, D.H. (1966a) Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J. Cell Sci. 1, 109–120Google Scholar
  13. Pickett-Heaps, J.D., Northcote, D.H. (1966b) Cell division in the formation of the stomatal complex of the young leaves of wheat. J. Cell Sci. 1, 121–128Google Scholar
  14. Schmit A.C., Vantard, M., de Mey, J., Lambert, A.M. (1983) Aster-like microtubule centers establish spindle polarity during interphase-mitosis transition in higher plant cells. Plant Cell Rep 2, 285–288Google Scholar
  15. Simmonds, D.H., Setterfield, G. (1986) Aberrant microtubule organization can result in genetic abnormalities in protoplast cultures of Vicia hajastana Grossh. Planta 167, 460–468Google Scholar
  16. Simmonds, D.H., Setterfield, G., Brown, D.L. (1983) Organization of microtubules in dividing and elongating cells of Vicia hajastana Grossh. in suspension culture. Eur. J. Cell Biol. 32, 59–66Google Scholar
  17. Wick, S.M., Duniec, J. (1983) Immunofluorescence microscopy of tubulin and microtubular arrays in plant cells. I. Preprophase band development and concomitant appearance of nuclear envelope-associate tubulin. J. Cell Biol. 97, 235–243Google Scholar
  18. Wick, S.M., Duniec, J. (1984) Immunofluorescence microscopy of tubulin and microtubular arrays in plant cells. II. Transition between the pre-prophase band and the mitotic spindle. Protoplasma 122, 45–55Google Scholar
  19. Williamson, F.A., Fowke, L.C., Weber, G., Constabel, F., Gamborg, O. (1977) Microfibril deposition on cultured protoplasts of Vicia hajastana. Protoplasma 91, 213–219Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • D. H. Simmonds
    • 1
  1. 1.Biology DepartmentCarleton UniversityOttawaCanada

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