Planta

, Volume 167, Issue 4, pp 425–436 | Cite as

Immunogold localization of nodule-specific uricase in developing soybean root nodules

  • K. A. Van den Bosch
  • E. H. Newcomb
Article

Abstract

Immunogold labeling was used to study the time of appearance and distribution of a nodule-specific form of uricase (EC 1.7.3.3) in developing nodules of soybean (Glycine max (L.) Merr.) inoculated with Bradyrhizobium japonicum. The enzyme was detected in thin sections of tissue embedded in either L R White acrylic resin or Spurr's epoxy resin, by employing a polyclonal antibody preparation active against a subunit of soybean nodule uricase. Antigenicity was better preserved in L R White resin, but ultrastructure was better maintained in Spurr's. Uricase was first detectable with protein A-gold in young, developing peroxisomes in uninfected cells, coincident with the release of Bradyrhizobium bacteroids from infection threads in adjacent infected cells. As the peroxisomes enlarged, labeling of the dense peroxisomal matrix increased. Gold particles were never observed over the paracrystalline inclusions of peroxisomes, however. Despite a close association between enlarging peroxisomes and tubular endoplasmic reticulum, uricase was not detectable in the latter. In mature nodules, labeling of uricase was limited to the large peroxisomes in uninfected cells. Small peroxisome-like bodies present in infected cells did not become labeled.

Key words

Bradyrhizobium Glycine (uricase in nodules) Immunogold labeling Nodulin Peroxisome biogenesis Root nodules Uricase 

Abbreviations

BSA

bovine serum albumin

Da

dalton

ER

endoplasmic reticulum

IgG

immunoglobulin G

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • K. A. Van den Bosch
    • 1
  • E. H. Newcomb
    • 1
  1. 1.Department of BotanyUniversity of WisconsinMadisonUSA

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