, Volume 152, Issue 2, pp 168–170 | Cite as

Determination of phytohormones in phloem exudate from tree species by radioimmunoassay

  • Elmar W. Weiler
  • Hubert Ziegler


A sensitive and specific radioimmunoassay was used to determine quantitatively four of the most important phytohormones in the phloem exudate from 14 different tree species of 8 genera. For cytokinins and indole-3-acetic acid (IAA) we found higher concentrations than those reported previously for other species. The gibberellin values were of the same order of magnitude as in earlier analyses (with different methods) of tree phloem exudates, but lower than the ones reported for Ricinus. Free abscisic acid (ABA) was found in tree phloem exudates in similar concentrations as before in Yucca or palm phloem exudate, but at considerably lower ones than reported for Ricinus and in Lupinus phloem exudate.

Key words

Abscisic acid Auxin Cytokinin Gibberellin Phloem exudate Radioimmunoassay (phytohormones) Trees (phytohormones) 



indole-3-acetic acid


abscisic acid


gibberellic acid


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Elmar W. Weiler
    • 1
  • Hubert Ziegler
    • 2
  1. 1.Lehrstuhl für PflanzenphysiologieRuhr-Universität BochumBochumFederal Republic of Germany
  2. 2.Institut für Botanik und MikrobiologieTechnische Universität MünchenMünchen 2Federal Republic of Germany

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