Abstract
Several live specimens of the blue-ringed octopus Octopus maculosus were collected from the Philippines in November 1985, and from Japan in February 1986, and the distribution of toxicity, along with toxin composition, in the posterior salivary gland and other soft parts were examined. Tetrodotoxin (TTX: 1400 mouse units g-1) was detected in the posterior salivary gland of a Japanese specimen, while not only the salivary gland but other soft parts were toxic in the Philippine specimens. The Philippine specimens contained TTX and anhydrotetrodotoxin, the Japanese specimen TTX, 4-epitetrodotoxin, and an unknown toxin. The posterior salivary gland, intestine and other parts were excised from the Philippine specimens and examined for bacterial flora. Twenty-two dominant strains were isolated and cultured in a 2xORI medium (Ocean Research Institute, Simidu and Tsukamoto 1985) at 20°C for 20 to 48 h. Cells were harvested by centrifugation, and disrupted by ultrasonication. The toxins were partially purified from the cell lyzate by ultrafiltration and Bio-Gel P-2 column-chromatography. Instrumental analyses disclosed that 16 of the 22 strains produced TTX and/or related substances. Six strains which clearly exhibited TTX productivity were identified as Alteromonas (2 strains), Bacillus (2), Pseudomonas (1) and Vibrio (1), based on biochemical and biological characteristics. Of these, one strain each of Bacillus and Pseudomonas produced TTX at a level detectable by the mouse assay.
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Freeman, S. E., Turner, R. J. (1970). Maculotoxin, a potent toxin secreted by Octopus maculosus Hoyle. Toxic. appl. Pharmac. 16: 681–690
Goto, T., Kishi, Y., Takahashi, S., Hirata, Y. (1965). Tetrodotoxin. Tetrahedron 21: 2059–2088
Halstead, B. W. (1978). Poisonous and venomous marine animals of the world. Darwin Press, Princeton, New Jersey
Howden, M. E. H., Williams, P. A. (1974). Occurrence of amines in the posterior salivary glands of the octopus Hapalochlaena maculosa (Cephalopoda). Toxicon 12: 317–320
Kadota, H., Taga, N. (1985). Methods in marine microbiology. Gakkai Shuppan Center, Tokyo
Kawabata, T. (1978). Food hygiene examination manual. Vol. 2. Assay method for tetrodotoxin. Japan Food Hygiene Association. Tokyo
Kim, Y. H., Brown, G. B., Mosher, H. S. (1975). Tetrodotoxin: occurrence in atelopid frogs of Costa Rica. Science, N.Y. 189: 151–152
Kungsuwan, A., Noguchi, T., Arakawa, O., Simidu, U., Tsukamoto, K., Shida, Y, Hashimoto, K. (in press). Tetrodotoxin-producing bacteria from the horseshoe crab Carcinoscorpius rotundicauda. Nippon Suisan Gakk.
Mosher, H. S., Fuhrman, F. A., Buchwald, H. D., Fisher, H. G. (1964). Tarichatoxin-tetrodotoxin: a potent neurotoxin. Science, N.Y. 144: 1100–1110
Nagashima, Y., Maruyama, J., Noguchi, T., Hashimoto, K. (1987). Analysis of paralytic shellfish poison and tetrodotoxin by ionpairing high performance liquid chromatography. Nippon Suisan Gakk. 53: 819–823
Narita, H., Matsubara, S., Miwa, N., Akahane, S., Murakami, M., Goto, T., Nara, M., Noguchi, T., Saito, T., Shida, Y., Hashimoto, K. (1987). Vibrio alginolyticus, a TTX-producing bacterium isolated from the starfish Astropecten polyacanthus. Nippon Suisan Gakk. 53: 617–621
Noguchi, T., Hwang, D. F., Arakawa, O., Sugita, H., Deguchi, Y., Shida, Y., Hashimoto, K. (1987). Vibrio alginolyticus, a tetrodotoxin-producing bacterium, in the intestines of the fish Fugu vermicularis vermicularis. Mar. Biol. 94: 625–630
Noguchi, T., Jeon, J. K., Arakawa, O., Sugita, H., Deguchi, Y., Shida, Y., Hashimoto, K. (1986). Occurrence of tetrodotoxin and anhydrotetrodotoxin in Vibrio sp. isolated from the intestines of a xanthid crab, Atergatis floridus. J. Biochem. Tokyo 99: 311–314
Noguchi, T., Maruyama, J., Ueda, Y., Hashimoto, K., Harada, T. (1981). Occurrence of tetrodotoxin in the Japanese ivory shell Babylonia japonica. Nippon Suisan Gakk. 47: 909–913
Pavelka, L. A., Kim, Y. H., Mosher, H. S. (1977). Tetrodotoxin and tetrodotoxin-like compounds from the eggs of the Costa Rican frog, Atelopus chiriquiensis. Toxicon 15: 135–139
Savage, I. V. E., Howden, M. E. H. (1977). Hapalotoxin, a second lethal toxin from the octopus Hapalochlaena maculosa. Toxicon 15: 463–466
Sheumack, D. D., Howden, M. E. H., Spence, I. (1984). Occurrence of a tetrodotoxin-like compound in the eggs of the venomous blue-ringed octopus (Hapalochlaena maculosa). Toxicon 22: 811–812
Sheumack, D. D., Howden, M. E. H., Spence, I., Quinn, R. J. (1978) Maculotoxin: a neurotoxin from the venom glands of the octopus Hapalochlaena maculosa identified as tetrodotoxin. Science, N.Y. 199: 188–189
Simidu, U., Noguchi, T., Hwang, D. F., Shida, Y., Hashimoto, K. (1987). Marine bacteria which produce tetrodotoxin. Appl. envirl. Microbiol. 53: 1714–1715
Simidu, U., Tsukamoto, K. (1985). Habitat segregation and biochemical activities of marine members of the family Vibrionaceae. Appl. envirl. Microbiol. 50: 781–790
Simon, S. E., Cairncross, K. D., Satchell, D. G., Gay, W. S., Edwards, S. (1964). The toxicity of Octopus maculosus Hoyle venom. Archs. int. Pharmacodyn. Thér. 149: 318–329
Sugita, H., Ueda, R., Noguchi, T., Arakawa, O., Hashimoto, K., Deguchi, Y. (1987). Identification of a tetrodotoxin-producing bacterium isolated from the xanthid crab Atergatis floridus. Nippon Suisan Gakk. 53: 1693
Tansill, B. (1984). Bergey's manual of systematic bacteriology, 1. Williams & Wilkins, Baltimore
Trethewie, E. R. (1965). Pharmacological effects of the venom of the common octopus Hapalochlaena maculosa. Toxicon 3: 55–59
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Communicated by M. Anraku, Tokyo
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Hwang, D.F., Arakawa, O., Saito, T. et al. Tetrodotoxin-producing bacteria from the blue-ringed octopus Octopus maculosus . Mar. Biol. 100, 327–332 (1989). https://doi.org/10.1007/BF00391147
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DOI: https://doi.org/10.1007/BF00391147