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Planta

, Volume 179, Issue 3, pp 409–413 | Cite as

Comparison of Site I auxin binding and a 22-kilodalton auxin-binding protein in maize

  • Alan M. Jones
  • Patrick Lamerson
  • Michael A. Venis
Article

Abstract

Several properties of a 43-kilodalton (kDa) auxin-binding protein (ABP) having 22-kDa subunits are shared by a class of auxin binding designated Site I. The spatial distribution of the ABP in the maize (Zea mays L.) mesocotyl corresponds with the distribution of growth induced by naphthalene-1-acetic acid and with the distribution of Site I binding as previously shown by J.D. Walton and P.M. Ray (1981, Plant Physiol. 68, 1334–1338). The greatest abundance of both ABP and Site I activity is at the apical region of the mesocotyl. The ABP and Site I activity co-migrate in isopycnic centrifugation with the endoplasmic-reticulum marker, cytochrome-c reductase. Red light, at low and high fluence, far-red and white light were used to alter the elongation rate of apical 1-cm sections of etiolated maize mesocotyls, the amount of auxin binding, and the abundance of the ABP. Relative changes in auxin binding and the ABP were correlated, but the growth rate was not always correlated with the abundance of the ABP.

Key words

Auxin binding Auxin-binding protein Zea (auxin binding) 

Abbreviations

ABP

auxin-binding protein

ER

endoplasmic reticulum

FR

far-red light

kDa

kilodalton

NAA

naphthalene-1-acetic acid

PM

plasma membrane

R

red light

SDS-PAGE

sodium dodecylsulfate-polyacrylamide gel electrophoresis

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Alan M. Jones
    • 1
  • Patrick Lamerson
    • 1
  • Michael A. Venis
    • 2
  1. 1.Department of BiologyUniversity of North CarolinaChapel HillUSA
  2. 2.Institute of Horticultural ResearchAgricultural and Food Research CouncilEast MallingUK

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