Molecular and General Genetics MGG

, Volume 242, Issue 2, pp 237–240 | Cite as

Deletion of the prc (tsp) gene provides evidence for additional tail-specific proteolytic activity in Escherichia coli K-12

  • Karen R. Silber
  • Robert T. Sauer
Short Communication


The Escherichia coli protease Prc (Tsp) exhibits specificity in vitro for proteins with nonpolar carboxyl termini. To determine whether Prc is responsible for the selective degradation in vivo of proteins with nonpolar carboxyl termini, we constructed a prc (tsp) deletion strain. Deletion of the prc gene did not prevent the rapid intracellular degradation of a variant of the amino-terminal domain of λ repressor with a nonpolar carboxyl terminus, even though this protein is a substrate for Prc in vitro. Our results indicate that at least one additional carboxy-terminal-specific proteolytic system must exist in E. coli.

Key words

Selective protein degradation prc (tsp) deletion strain Carboxyl terminus λ repressor Escherichia coli K-12 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Karen R. Silber
    • 1
  • Robert T. Sauer
    • 1
  1. 1.Department of Biology, Room 16-843Massachusetts Institute of TechnologyCambridgeUSA
  2. 2.ScripTech Pharmaceuticals Inc.CambridgeUSA

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