Uptake of auxotrophic cells of a heterocyst-forming cyanobacterium by tobacco protoplasts, and the fate of their associations
Article
Received:
Accepted:
- 55 Downloads
- 17 Citations
Abstract
Auxotrophic cells of the filamentous cyanobacterium Anabaena variabilis Kütz. were introduced into protoplasts of Nicotiana tabacum L. in an attempt to engineer a nitrogen-fixing endosymbiosis. Conditions were established to maximize uptake of the cyanobacteria by use of polyethylene glycol. Culture of the novel association was not successful: tobacco protoplasts with Anabaena inside did not appear to divide. Most of the protoplasts expelled the cyanobacteria and simultaneously disintegrated. The reasons for the incompatibility are not known.
Key words
Anabaena Cell uptake Cyanobacterium Nicotiana Polyethylene glycol ProtoplastPreview
Unable to display preview. Download preview PDF.
References
- Allen, M.B., Arnon, D.I.: Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiol. 30, 366–372 (1955)Google Scholar
- Bonnett, H.T.: On the mechanism of the uptake of Vaucheria chloroplasts by carrot protoplasts treated with polyethylene glycol. Planta 131, 229–233 (1976)Google Scholar
- Bonnett, H.T., Eriksson, T.: Transfer of algal chloroplasts into protoplasts of higher plants. Planta 120, 71–79 (1974)Google Scholar
- Bonnett, H.T., Banks, M.S.: Chloroplast incorporation, survival, and replication in foreign cytoplasm. In: International Cell Biology, p. 225–231. Brinkley, B.R., Porter, K.R., eds. New York: Rockefeller University Press 1976–1977Google Scholar
- Burgoon, A.C., Bottino, P.J.: Uptake of the nitrogen fixing bluegreen algae Gloeocapsa into protoplasts of tobacco and maize. J. Hered. 67, 223–226 (1976)Google Scholar
- Currier, T.C., Haury, J.H., Wolk, C.P.: Isolation and preliminary characterization of auxotrophs of a filamentous cyanobacterium. J. Bacteriol. 129, 1556–1562 (1977)PubMedGoogle Scholar
- Davey, M.R., Cocking, E.C.: Uptake of bacteria by isolated higher plant protoplasts. Nature 239, 455–456 (1972)PubMedGoogle Scholar
- Davey, M.R., Power, J.B.: Polyethylene glycol-induced uptake of microorganisms into higher plant protoplasts: an ultrastructural study. Plant Sci. Lett. 5, 267–274 (1975)Google Scholar
- Dilworth, M.J.: Dinitrogen fixation. Ann. Rev. Plant Physiol. 25, 81–114 (1974)CrossRefGoogle Scholar
- Giles, K.L., Whitehead, H.C.M.: The transfer of nitrogen fixing ability to a eukaryote cells. Cytobios 14, 49–61 (1975)Google Scholar
- Giles, K.L., Whitehead, H.C.M.: Uptake and continued metabolic activity of Azotobacter within fungal protoplasts. Science 193, 1125–1126 (1976)Google Scholar
- Kao, K.N., Constabel, F., Michayluk, M.R., Gamborg, O.: Plant protoplast fusion and growth of intergeneric hybrid cells. Planta 120, 215–227 (1974)Google Scholar
- Kao, K.N., Michayluk, M.R.: A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115, 355–367 (1974)Google Scholar
- Linsmaier, E.M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures. Physiol. Plantarum 18, 100–127 (1965)Google Scholar
- Nagata, T., Takebe, I.: Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99, 12–20 (1971)Google Scholar
- Uchimiya, H., Murashige, T.: Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells. Plant Physiol. 54, 936–944 (1974)Google Scholar
- Wolk, C.P., Shaffer, P.W.: Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium. Arch. Microbiol. 110, 145–147 (1976)PubMedGoogle Scholar
- Wolk, C.P., Wojciuch, E.: Simple methods for plating single vegetative cells of, and for replica-plating, filamentous blue-green algae. Arch. Microbiol. 91, 91–95 (1973)Google Scholar
Copyright information
© Springer-Verlag 1978