Planta

, Volume 117, Issue 2, pp 123–132 | Cite as

Carbon dioxide fixation by epidermal and mesophyll tissues of Tulipa and Commelina

  • C. M. Willmer
  • P. Dittrich
Article

Summary

Rates of 14CO2 fixation by epidermal tissue of Tulipa gesneriana (tulip) and Commelina diffusa are only slightly higher in the light than in the dark while in mesophyl tissues rates are much greater in the light. The first products of 14CO2 fixation by epidermal tissue of Tulipa gesneriana and C. diffusa in the light and dark are malate and aspartate. In addition to these dominating dicarboxylic acids, 3-phosphoglyceric acid and sugar phosphates appear in the light, while in the dark only the amino acids, glutamate and glutamine become labelled. Mesophyll tissue of tulip and C. diffusa, however, gives typical CO2 fixation patterns of the labelled products of C3 plants. Furthermore, a period of dark 14CO2 fixation followed by a light 12CO2 chase carried out with epidermal tissue suggested that malate can act has the precursor of phosphorylated compounds of the Calvin cycle and consequently of starch. The data are consistent with the view that guard cells are able to exhibit Crassulacean acid metabolism.

Abbreviations

MDH

malate dehydrogenase

PEP

phosphoenolpyruvate

RuDP

ribulose-1,5-diphosphate

TCA

tricarboxylic acid

CAM

crassulacean acid metabolism

OAA

oxaloacetate

PGA

phosphoroglyceric acid

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References

  1. Allaway, W. G.: Accumulation of malate in guard cells of Vicia faba L. during stomatal opening. Planta (Berl.) 110, 63–70 (1973)Google Scholar
  2. Arisz, W. H.: Influx and efflux by leaves of Vallisneria spiralis. 1. Active uptake and permeability. Protoplasma (Wien) 57, 5–26 (1963)Google Scholar
  3. Bassham, J. A., Calvin, M.: The path of carbon in photosynthesis. 104 pp. Englewood Cliffs, N. J.: Prentice Hall, Inc. 1957Google Scholar
  4. Glinka, Z., Reinhold, L.: Rapid changes in permeability of cell membranes to water brought about by carbon dioxide and oxygen. Plant Physiol. 37, 481–486 (1962)Google Scholar
  5. Hatch, M. D., Slack, C. R.: A new enzyme for the interconversion of pyruvate and phosphopyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis. Biochem. J. 106, 141–146 (1968)Google Scholar
  6. Humble, G. D., Raschke, K.: Stomatal opening quantitatively related to potassium transport. Evidence from electron probe analysis. Plant Physiol. 48, 447–453 (1971)Google Scholar
  7. Meidner, H., Mansfield, T. A.: Physiology of stomata, p. 73–76. London: McGraw-Hill 1968Google Scholar
  8. Rouhani, I., Vines, H. M., Black, C. C., Jr.: Isolation of mesophyll cells from Sedum telephium leaves. Plant. Physiol. 51, 97–103 (1973)Google Scholar
  9. Willmer, C. M., Kanai, R., Pallas, J. E., Jr., Black, C. C. Jr.: Detection of high levels of phosphoenolpyruvate carboxylase in leaf epidermal tissue and its significance in stomatal movements. Life Sci. 12, 151–155 (1973a)Google Scholar
  10. Willmer, C. M., Pallas, J. E., Jr., Black, C. C., Jr.: Carbon dioxide metabolism in leaf epidermal tissue. Plant Physiol. In press (1973b)Google Scholar
  11. Wintermans, J. F. G. M., De Mots, A.: Spectrophotometric characteristics of chlorophyll and their pheophytins in ethanol. Biochim. biophys. Acta (Amst.) 109, 448–453 (1965)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • C. M. Willmer
    • 1
  • P. Dittrich
    • 1
  1. 1.Department of BiologyUniversity of StirlingStirlingUK
  2. 2.Botanical InstituteUniversity of MunichMunichFederal Republic of Germany

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