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Excretion of modified nucleosides during development of malignant lymphomas in mice after whode body irradiation

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Summary

During x-ray-induced development of malignant lymphomas in mice their urinary excretion of eight modified nucleosides was monitored and the values were compared to the results of the histological examination of the animals at time of their sacrifice.

It was found that the pathologically augmented excretion of modified nucleosides begins as much as several weeks before the malignant lymphomas can be diagnosed clinically. Thus some mice had increased levels of modified nucleosides even 10 weeks before sacrifice, though at the time of sacrifice the histological investigation reveled only some small foci of reticulum cell neoplasm in their spleen.

It is therefore stressed that the usefulness of the determination of urinary modified nucleosides as an early noninvasive screening test for cancer in man and as an in vivo carcinogenicity test should be evaluated.

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Abbreviations

φ:

pseudouridine

m1A:

1-methyladenosine

m5C:

5-methylcytidine

m1I:

1-methylinosine

m1G:

1-methylguanosine

m2G:

N 2-methylguanosine

m2G:

N 2,N2-dimethylguanosine

ac4C:

N 4-acetylcytidine

mt6A:

N-(9-β-d-ribofuranosylpurine-6-yl)N-methylcarbamoyl) threonine

acp3U:

3-(3-amino-3-carboxypropyl) uridine

SD:

standard deviation

References

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Correspondence to Gisela Nass.

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Thomale, J., Luz, A. & Nass, G. Excretion of modified nucleosides during development of malignant lymphomas in mice after whode body irradiation. J Cancer Res Clin Oncol 108, 302–307 (1984). https://doi.org/10.1007/BF00390462

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Key words

  • Modified nucleosides
  • Tumor development
  • Leukemia in mice
  • Cancer-screening test