Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Production of gibberellin-like substance in the embryo of barley during germination

  • 58 Accesses

  • 41 Citations


Embryos were excised from barley seeds, their homogenates were incubated with ficin, and their content in gibberellin-like substance was assayed by means of α-amylase-producing activity, but no gibberellin-like substance could be detected.

Embryos free from endosperm which were cultured for five days produced measurable amounts of gibberellin-like substance. This substance was not produced when a carbon source was absent from the medium, or when air was removed after 2 days of aerobic culture. CCC and Phosfon D (at the concentration of 2×10-4 M and 0.8×10-4 M, respectively) inhibited the formation of the gibberellin-like substance in cultured embryos without affecting their growth.

Mevalonic acid could be used as a carbon source in the culture of the embryos. The formation of the gibberellin-like substance was in this case inhibited by 0.8×10-4 M Phosfon D, but was not inhibited by 2×10-3 M CCC.

This is a preview of subscription content, log in to check access.


  1. Birch, A. J., W. R. Richards, and H. Smith: The biosynthesis of gibberellic acid. Proc. Chem. Soc. 1958, 192–193.

  2. Dennis, D. T., C. D. Upper, and C. A. West: An enzymic site of inhibition of gibberellin biosynthesis by Amo 1618 and other growth retardants. Plant Physiol. 40, 948–952 (1965).

  3. Jones, D. F.: Examination of gibberellin of Zea mays and Phaseolus multiflorus using thin-layer chromatography. Nature (Lond.) 202, 1309–1310 (1964).

  4. Kende, H., H. Ninnemann, and A. Lang: Inhibition of gibberellic acid biosynthesis in Fusarium moniliforme by Amo 1618 and CCC. Naturwissenschaften 50, 599–600 (1963).

  5. MacComb, A. J.: “Bound” gibberellin in mature runner bean seeds. Nature (Lond.) 192, 575–576 (1961).

  6. Ninnemann, H., J. A. D. Zeevaart, H. Kende, and A. Lang: The plant growth retardant CCC as inhibitor of gibberellin biosynthesis in Fusarium moniliforme. Planta (Berl.) 61, 229–235 (1964).

  7. Paleg, L. G.: Physiological effect of gibberellic acid. II. On starch hydrolysing enzymes of barley endosperm. Plant Physiol. 35, 902–906 (1960).

  8. Yomo, H.: Studies on amylase activating substance. (5) Purification of the amylase activating substance in barley malt and its properties [In Japanese]. Hakko Kyokai Shi 18, 603–606 (1960).

  9. —, and H. Iinuma: Activation of enzymes in ungerminated barley endosperms with gibberellin. Agric. and Biol. Chem. 27, 76–79 (1963).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yomo, H., Iinuma, H. Production of gibberellin-like substance in the embryo of barley during germination. Planta 71, 113–118 (1966). https://doi.org/10.1007/BF00390130

Download citation


  • Carbon Source
  • Measurable Amount
  • Barley Seed
  • Mevalonic Acid
  • Aerobic Culture