, Volume 134, Issue 1, pp 91–93 | Cite as

Development of maize caryopses resulting from in-vitro pollination

  • B. G. Gengenbach
Short Communication


Intact maize (Zea mays L.) ovaries were excised from unpollinated ears (pistillate inflorescences of field-grown plants and placed on defined, agar-based media in Petri dishes. Application of pollen to the end of silks (styles) positioned outside the Petri dish resulted in fertilization of 46% of the ovaries. The extent of subsequent kernel (caryopsis) development varied. After 40 days some kernels had only embryo development while others had embryo and variable endosperm development. About 5% of the initial ovaries developed into normal kernels; 60% of the kernels with some endosperm germinated under laboratory conditions, and 70% of the embryos excised from the embryo-only kernels germinated on culture media.

Key words

Embryo development in vitro Fertilization in vitro In vitro pollination Pollination in vitro Seeddevelopment in vitro Zea 


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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • B. G. Gengenbach
    • 1
  1. 1.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA

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