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Planta

, Volume 129, Issue 2, pp 183–189 | Cite as

Biosynthesis and release of cell wall-like glycoproteins during the vegetative cell cycle of Chlamydomonas reinhardii

  • Winfried C. Lang
  • Maarten J. Chrispeels
Article

Summary

The culture medium of asynchronously growing Chlamydomonas reinhardii cells contains distinct proteins which are derived from the cell walls of these cells. When cultures are synchronized by a light-dark cycle cell wall proteins are synthesized throughout the cycle, but the release of these proteins into the culture medium occurs primarily in the last quarter of the cycle, after cell separation has occurred. The mutant CW-2, which does not form a normal cell wall, continuously synthesizes and secretes cell wall proteins into the culture medium. The synthesis of cell wall protein during the cell cycle appears to be modulated and peaks of synthesis occur at the end of the light period and in the second half of the dark period, shortly after cell separation. At these times the cells devote 15% of their protein-synthetic capacity to making cell wall proteins.

Keywords

Cell Cycle Cell Wall Normal Cell Vegetative Cell Chlamydomonas 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

SDS

sodium dodecyl sulfate

TCA

trichloroacetic acid

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References

  1. 1.
    Albersheim, P., Nevins, D.J., English, P.D., Karr, A.: A method for the analysis of sugars in plant cell wall polysaccharides by gas-liquid chromatography. Carbohyd. Res. 5, 340–345 (1967)CrossRefGoogle Scholar
  2. 2.
    Chrispeels, M.J.: Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Kinetic analysis. Plant Physiol. 44, 1187–1193 (1969)Google Scholar
  3. 3.
    Davies, D.R., Lyall, V.: The assembly of a highly ordered component of the cell wall: The role of heritable factors and of physical structure. Molec. Gen. Genet. 124, 21–34 (1973)CrossRefPubMedGoogle Scholar
  4. 4.
    Davies, D.R., Plaskitt, A.: Genetical and structural analysis of cell wall formation in Chlamydomonas reinhardii. Genet. Res. 17, 33–43 (1971)Google Scholar
  5. 5.
    Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances. An. Chem. 28, 350–356 (1956)Google Scholar
  6. 6.
    Howell, S.H.: An analysis of cell cycle controls in temperature sensitive mutants of Chlamydomonas reinhardii. Cell Cycle Controls, p. 235–249. Padilla, G.M., Cameron, I.L., Zimmerman, A., (eds.) New York: Academic Press 1974Google Scholar
  7. 7.
    Jones, R.F., Kates, J.F., Keller, S.J.: Protein turnover and macromolecular synthesis during growth and gametic differentiation in Chlamydomonas reinhardii. Biochim. biophys. Acta AAmst.) 157, 589–598 (1968)Google Scholar
  8. 8.
    Kates, J.R., Jones, R.F.: Periodic increase in enzyme activity in synchronized cultures of Chlamydomonas reinhardii. Biochim. biophys. Acta (Amst.) 145, 153–158 (1967)Google Scholar
  9. 9.
    Kivirriko, K.J.: Hydroxyproline containing fractions in normal and cortisone treated chick embryo. Acta. Physiol. Scand. 60, suppl. 219 (1963)Google Scholar
  10. 10.
    Miller, D.H., Lamport, D.T.A., Miller, M.: Hydroxyproline hetero-oligosaccharides in Chlamydomonas. Science 176, 918–920 (1972)PubMedGoogle Scholar
  11. 11.
    Moore, S., Stein, W.H.: Chromatographic determination of amino acids by the use of automatic recording equipment. Methods in Enzymology 6, p. 819–831, Eds.: Colowick, S.P., Kaplan, N.O., New York: Academic Press 1963Google Scholar
  12. 12.
    Roberts, K.: Crystalline glycoprotein cell wall of algae: Their structure, composition and assembly. Phil. Trans. roi. Soc. Lond. B. 129–146 (1974)Google Scholar
  13. 13.
    Spies, J.R.: Colorimetric procedures for amino acids. II. Ninhydrin method. Methods in Enzymology 3, pp. 468–471, Eds.: Colowick, S.P., Kaplan, N.O. New York: Academic Press 1957Google Scholar
  14. 14.
    Sueoka, N., Chiang, K.S., Kates, J.R.: Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardii. J. molec. Biol. 25, 47 (1967)PubMedGoogle Scholar
  15. 15.
    Surzycki, S.: Synchronously grown cultures of Chlamydomonas reinhardii. Methods in Enzymology 23, p. 67–73, Ed.: San Pietro, A., New York: Academic Press 1971Google Scholar
  16. 16.
    Weber, K., Osborn, M.: The reliability of molecular weight determination by dodecyl sulfate polyacrylamide gel electrophoresis. Biol. Chem. 244, 4406–4412 (1969)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Winfried C. Lang
    • 1
  • Maarten J. Chrispeels
    • 1
  1. 1.Department of Biology, John Muir CollegeUniversity of CaliforniaLa JollaUSA

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