Bioprocess Engineering

, Volume 9, Issue 4, pp 147–154 | Cite as

Simulation of a multicolumn recirculated packed bed reactor (MRPBR) for penicillin acylase

  • A. Gomez Aguirre
  • R. Quintero
  • A. Lopez-Munguia
Originals

Abstract

Enzyme reactors for the industrial hydrolysis of penicillin are analyzed in terms of biocatalyst stability to pH. A multicolumn system with packed beds placed in parallel and operating under recirculating conditions is proposed as an adequate reactor for this process. The system is studied both experimentally and with the aid of a simulation program.

List of Symbols

A

transversal area (cm2)

CA

ammonia concentration in the reaction mixture (M)

C1

concentration of KH2PO4 in buffer (M)

C2

concentration of K2HPO4 in buffer (M)

dp

biocatalyst diameter (cm)

E

enzyme or biocatalyst concentration (gcat l−1)

KAPA

APA non competitive inhibition constant (M)

KIS

excess substrate inhibition constant (M)

Km

constant Michaelis-Menten (M)

KPAA

PAA competitive inhibition constant (M)

Q

recirculation flow rate (cm3 min−1)

QT

recirculation flow rate per column (cm3 min−1)

Re

Reynolds number

SE

substrate concentration entering the neutralization tank (M)

S0

initial substrate concentration (M)

ST

substrate concentration in neutralization tank (M)

t

time (min)

vi

initial reactor rate (mol min−1 gcat−1)

Vs

superficial velocity (cm seg−1)

VT

volume of neutralization tank (cm3)

XE

substrate conversion entering tank

XT

substrate conversion in neutralization tank

X

conversion

Z

reactor length (cm)

z

axial position in reactor (cm)

z*

non-dimensional axial position in reactor

ρ

biocatalyst's density (gcat cm−3)

Δp

pressure drop in the packed-bed reactor

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Poulsen, P.; Current application of immobilized enzymes for manufacturing purposes. Biotechnol. and Genetic Engineering Reviews (1984) 121–140Google Scholar
  2. 2.
    Ospina, S.: López-Munguia, A.: González, R.; Quintero, R.: Characterization and use of a penicillin acylase catalyst. J. Chem. Tech. Biotechnol. 53 (1992) 205–214.Google Scholar
  3. 3.
    Gómez, A. A.; Ospina, S.; Quintero, R.; López-Munguia, C. A.: Modelling and simulation of a pH dependent bioprocess: Enzymatic conversion of penicillin G to 6-APA. In: Ghose T. (Ed.) Bioprocess Computation in Biotechnology. Vol. II. United Kingdom, Ellis Horwood. In pressGoogle Scholar
  4. 4.
    Shewale, J.; Kumar, K.; Ambekar, K.; Evaluation or determination of 6-aminopenicillanic acid by p-dimethyl aminobenzaldehide. Biotechnol. Tech. 1 (1987) 69–72Google Scholar
  5. 5.
    Anon., Production of 6-APA from penicillin V in a stirred batch systems using semacylase TM from NOVO Industry A/S. IB. No. 236d-6B, Novo Enzyme Information. Denmark, 1987.Google Scholar
  6. 6.
    Picher, W.: Design and operation of immobilized enzyme reactors. In Immobilized Enzymes for Industrial Reactors. Edited by Ralpth A. Messing. Academic Press, N.Y. (1975) 151–199Google Scholar
  7. 7.
    McCabe W.L.: Smith J.C.: Unit operations of chemical engineering. Tokyo, Japan, McGraw-Hill Book Co. 1967Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • A. Gomez Aguirre
    • 1
  • R. Quintero
    • 2
  • A. Lopez-Munguia
    • 2
  1. 1.Instituto de BiotecnologiaUniversidad Nacional de ColombiaBogotaColombia
  2. 2.Instituto de BiotecnologiaUniversidad Nacional Autonoma de MexicoCuernavaca, MorelosMexico

Personalised recommendations