Urological Research

, Volume 24, Issue 6, pp 367–373 | Cite as

Experimental autoimmune cystitis in the Lewis rat: a potential animal model for interstitial cystitis

  • J. Luber-Narod
  • T. Austin-Ritchie
  • B. Banner
  • C. HollinsIII
  • C. Maramag
  • H. Price
  • M. Menon
Original Paper

Abstract

To develop an autoimmune animal model for interstitial cystitis (IC), we injected rats with Freund's adjuvant (CFA) containing bladder homogenate (experimentals) or CFA alone (shams). We observed a doubling of urinary frequency in the experimental animals over the shams (P=0.004) and histopathologic changes (venular congestion) consistent with IC. Statistically significant bladder capacity changes were not found. Mast cell (MC) number was not statistically different between experimentals and controls but the number of MCs from section to adjacent section within the same animal's bladder did vary markedly, indicating that MC counts are not a reliable measure of disease in the rat bladder. Splenocytes cultured from the experimental animals and transferred to naive syngeneic recipients were capable of transferring the urinary frequency changes and vascular congestion while splenocytes from animals which did not develop the condition were without effect. In summary, we have developed an autoimmune model for IC consistent with the clinical features of IC. The features of this model can be transferred to naive syngeneic recipients via adoptive splenocyte transfer. The model will permit us to ask and answer important questions about the pathogenesis and treatment of the human disease.

Key words

Interstitial cystitis Animal model Urinary frequency Venular congestion 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • J. Luber-Narod
    • 1
  • T. Austin-Ritchie
    • 2
  • B. Banner
    • 3
  • C. HollinsIII
    • 4
  • C. Maramag
    • 1
  • H. Price
    • 5
  • M. Menon
    • 1
  1. 1.Division of Urologic and Transplantation Surgery, Department of SurgeryUniversity of Massachusetts Medical SchoolWorcesterUSA
  2. 2.Cambridge BioscienceWorcesterUSA
  3. 3.Department of PathologyUniversity Massachusetts Medical SchoolWorcesterUSA
  4. 4.BASFWorcesterUSA
  5. 5.Division of UrologyUniversity of Texas-Houston Medical SchoolHoustonUSA

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