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Studies on immobilization of lipase on alumina for hydrolysis of ricebran oil

Abstract

The immobilization of lipase from Candida lipolytica on alumina by adsorption for the hydrolysis of ricebran oil is described. Some of the factors which influence the activity of immobilizate and the stability of immobilizate are discussed. About 69% of the initially added enzyme activity is found in the biocatalyst when immobilized at pH 7.2. The stability of the immobilized enzyme to different pH and temperature conditions has also been studied.

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Abbreviations

DE:

Dextrose equivalent. One DE is reducing sugar equivalent to one milligram of glucose

NE:

Nitrogen equivalent. One NE is one milligram of nitrogen

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Correspondence to Prof. A. Baradarajan.

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Padmini, P., Rakshit, S.K. & Baradarajan, A. Studies on immobilization of lipase on alumina for hydrolysis of ricebran oil. Bioprocess Engineering 9, 43–46 (1993). https://doi.org/10.1007/BF00389539

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Keywords

  • Enzyme
  • Alumina
  • Hydrolysis
  • Waste Water
  • Enzyme Activity