Planta

, Volume 140, Issue 1, pp 89–92 | Cite as

Growth of Avena coleoptiles and pH drop of protoplast suspensions induced by chlorinated indoleacetic acids

  • Michael Böttger
  • Kjeld C. Engvild
  • H. Soll
Article
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Accepted:

Abstract

Several indoleacetic acids, substituted in the benzene ring, were compared in the Avena straight growth bioassay. 4-Chloroindoleacetic acid, a naturally occurring plant hormone, is one of the strongest hormones in this bioassay. With an optimum at 10-6 mol l-1, it is more active than indoleacetic acid, 2,4-dichlorphenoxyacetic acid and naphthaleneacetic acid. 5-Chloro- and 6-chloroindoleacetic acids are very strong auxins as well. Other derivatives tested have a lower activity. 5,7-Dichloro- and 5-hydroxyindoleacetic acids have very low auxin activity at 10-4 mol l-1 and may be anti-auxins. Some of the derivatives were compared for their effect on pH decline in stem protoplast suspensions of Helianthus annuus L. and Pisum sativum L. The change of pH occurs without a lag period or with only a very short one. Derivatives which are very active in the Avena straight growth assay cause a larger pH decline than indoleacetic acid, while inactive derivatives cause effectively no pH decline.

Key words

Auxin Avena Helianthus pH drop Pisum Protoplast suspension 

Abbreviations

IAA

Indoleacetic acid

4-Cl-IAA

4-chloroindoleacetic acid

5,7-Cl2-IAA etc

5,7-dichloroindoleacetic acid

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References

  1. Bayer, M., Sonka, J.: The effect of indoleacetic acid on pH in mesophyll protoplast suspension of Vicia faba. Z. Pflanzenphysiol. 78, 271–276 (1976)Google Scholar
  2. Cleland, R.E.: Kinetics of hormone-induced H+ excretion. Plant Physiol. 58, 210–213 (1976)Google Scholar
  3. Durand, H., Rayle, D.L.: Physiological evidence for auxin-induced hydrogen-ion secretion and the epidermal paradox. Planta 114, 185–193 (1973)Google Scholar
  4. Edwards, K.L., Scott, T.K.: Rapid growth responses of corn root segments: effects of pH on elongation. Planta 119, 27–37 (1974)Google Scholar
  5. Hager, H., Menzel, H., Krauss, A.: Versuche und Hypothese zur Primärwirkung des Auxins beim Streckungswachstum. Planta 100, 47–75 (1971)Google Scholar
  6. Haschke, J.-P., Lüttge, U.: β-Ondolylessigsäure-(IES)-abhängiger K+-H+-Austauschmechanismus und Streckungswachstum bei Avena Koleoptilen. Z. Naturforsch. 28, 555–558 (1973)Google Scholar
  7. Gandar, J.-C., Nitsch, J.P.: Extraction d'une substance de croissance a partir des jeunes graines de Pisum sativum. In: Regulateurs naturels de la croissance végétale, pp. 169–178. Nitsch, J.P., ed. C.N.R.S. No. 123, Paris 1964Google Scholar
  8. Gandar, J.C., Nitsch, C.: Isolement de l'ester méthylique d'un acide chloro-3-indolylacetique à partir de graines immatures de pois, Pisum sativum L: C. R. Acad. Sci. (Paris) Ser. D. 265, 1795–1798 (1967)Google Scholar
  9. Hoffmann, O.L., Fox, S.W., Bullock, M.W.: Auxin-like activity of systematically substituted indoleacetic acid. J. Biol. Chem. 196, 437–441 (1952)PubMedGoogle Scholar
  10. Han, I.: On auxin pH drop and on the improbability of its involvement in the primary mechanism of auxin-induced growth promotion. Physiol. Plant. 28, 146–148 (1973)Google Scholar
  11. Marumo, S., Hattori, H., Abe, H., Munakata, K.: Isolation of 4-chloroindolyl-3-acetic acid from immature seeds of Pisum sativum. Nature 219, 959–960 (1968)Google Scholar
  12. Marumo, S., Hattori, H., Yamanoto, A.: Biological activity of 4-chloroindolyl-3-acetic acid. In: Plant growth substances pp. 419–428, Tokyo: Hirokawa 1973Google Scholar
  13. Muir, R.M., Hansch, C.: On the mechanism of action of plant growth regulators. Plant Physiol. 28, 218–232 (1953)Google Scholar
  14. Penny, P., Dunlop, J., Perley, J.E., Penny, D.: pH and auxin-induced growth: a causal relationship? Plant Sci. Lett. 4, 35–40 (1975)Google Scholar
  15. Perley, J.E., Penny, D., Penny, P.: A difference between auxin-induced and hydrogen ion-induced growth. Plant Sci. Lett. 4, 133–136 (1975)CrossRefGoogle Scholar
  16. Porter, W.L., Thimann, K.V.: Molecular requirements for auxin action. I. Halogenated indoles and indoleacetic acid. Phytochemistry 4, 229–243 (1965)CrossRefGoogle Scholar
  17. Sell, H.M., Wittwer, S.H., Rebstock, T.L., Redemann, C.T.: Comparative stimulation of parthenocarpy in the tomato by various indole compounds. Plant Physiol. 28, 481–487 (1963)Google Scholar
  18. Vanderhoef, L.N., Shen, Lu. T.-Y., Williams, C.A.: Comparison of auxin-induced elongation in soybean hypocotyl. Plant Physiol. 59, 1004–1007 (1977)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Michael Böttger
    • 1
  • Kjeld C. Engvild
    • 2
  • H. Soll
    • 1
  1. 1.Institut für Allgemeine Botanik der UniversitätHamburg 36Federal Republic of Germany
  2. 2.Agricultural Research DepartmentRisø National LaboratoryRoskildeDenmark

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