The distribution of glyoxylate-cycle enzymes between microbodies and mitochondria was examined in ethanol-grown Aspergillus tamarii Kita. Particulate activities of catalase and the two glyoxylate by-pass enzymes, malate synthase and isocitrate lyase, were localized in the microbodies. The microbodies had a buoyant density of about 1.23 g cm-3 after isopycnic centrifugation in linear sucrose gradients. Particulate activities of the other two glyoxycitrate synthase, together with that of succinate dehydrogenase were restricted to the mitochondria, which had a buoyant density of about 1.20 g cm-3. Catalase also appeared to be localized in a second particle, perhaps the microbody inclusions or the Woronin bodies, having a buoyant density of about 1.26 g cm-3.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Beevers, H.: Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann. N.Y. Acad. Sci. 168, 313–324 (1969)
Burke, J.J., Trelease, R.N.: Cytochemical demonstration of malate synthase and glycolate oxidase in microbodies of cucumber cotyledons. Plant Physiol. 56, 710–717 (1975)
Camp, R.R., Whittingham, W.F.: Host-parasite relationships in sooty blotch disease of white clover. Amer. J. Bot. 59, 1057–1067 (1972)
Cooper, T.G., Beevers, H.: Mitochondria and glyoxysomes from castor bean endosperm. J. biol. Chem. 244, 3507–3513 (1969)
Flavell, R.B., Fincham, J.R.S.: Acetate-nonutilizing mutants of Neurospora crassa. II. Biochemical deficiencies and the role of certain enzymes. J. Bact. 95, 1063–1068 (1968)
Flavell, R.B., Woodward, D.O.: Metabolic role, regulation of synthesis, cellular localization, and genetic control of the glyoxylate cycle enzymes in Neurospora crassa. J. Bact. 105, 200–210 (1971)
Frederick, S.E., Newcomb, E.H.: Cytochemical localization of catalase in leaf microbodies (peroxisomes) J. Cell Biol. 43, 343–353 (1969)
Frederick, S.E., Newcomb, E.H.: Ultrastructure and distribution of microbodies in leaves of grasses with and without CO2-photorespiration. Planta (Berl.) 96, 152–174 (1971)
Graves, L.B., Jr., Trelease, R.N., Grill, A., Becker, W.M.: Localization of glyoxylate cycle enzymes in glyoxysomes in Euglena. J. Protozool. 19, 527–532 (1972)
Grove, S.N., Bracker, C.E., Morré, D.J.: An ultrastructural basis for hyphal tip growth in Pythium ultimum. Amer. J. Bot. 57, 245–266 (1970)
Hänssler, G., Maxwell, D.P., Maxwell, M.D.: Demonstration of acid phosphatase-containing vacuoles in hyphal tip cells of Sclerotium rolfsii. J. Bact. 124, 997–1006 (1975)
Kobr, M.J., Vanderhaeghe, F.: Changes in density of organelles from Neurospora. Experientia 29, 1221–1223 (1973)
Kobr, M.J., Vanderhaeghe, F., Combépine, G.: Particulate enzymes of the glyoxylate cycle in Neurospora crassa. Biochem. Biophys. Res. Commun. 37, 640–645 (1969)
Laborda, F., Maxwell, D.P.: Ultrastructural changes in Cladosporium cucumerinum during pathogenesis. Canad. J. Microbiol. 22, 394–403 (1976)
Lowry, O.H., Rosebrough, N.J., Farr, A.J., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Lück, H.: Catalase. In: Methods of enzymatic analysis. pp. 885–894, Ed.: Bergemeyer, H.U. New York: Academic Press 1963
Maxwell, D.P., Lumsden, R.D.: Oxalic acid production by Sclerotinia sclerotiorum in infected bean and in culture. Phytopathology 60, 1395–1398 (1970)
Maxwell, D.P., Maxwell, M.D., Hänssler, G., Armentrout, V.N., Murray, G.M., Hoch, H.C.: Microbodies and glyoxylate-cycle enzyme activities in filamentous fungi. Planta (Berl.) 124, 109–123 (1975)
Maxwell, D.P., Williams, P.H., Maxwell, M.D.: Studies on the possible relationships of microbodies and multivesicular bodies to oxalate, endopolygalacturonase, and cellulase (Cx) production by Sclerotinia sclerotiorum. Canad. J. Bot. 50, 1743–1748 (1972)
Mills, G.L., Cantino, E.C.: Isolation and characterization of microbodies and symphyomicrobodies with different buoyant densities from the fungus Blastocladiella emersonii. Biochem. Biophys. Res. Commun. 67, 1256–1263 (1975)
Müller, M., Hogg, J.F., De Duve, C.: Distribution of tricarboxylic acid cycle enzymes and glyoxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis. J. biol. Chem. 243, 5385–5395 (1968)
O'Sullivan, J., Casselton, P.J.: The subcellular localization of glyoxylate cycle enzymes in Coprinus lagopus (sensu Buller). J. Gen. Microbiol. 75, 333–337 (1973)
Parish, R.W.: The lysosome-concept in plants. II. Localization of acid hydrolases in maize root tips. Planta (Berl.) 123, 15–31 (1975)
Roggenkamp, R., Sahm, H., Hinkelmann, W., Wagner, F.: Alcohol oxidase and catalase in peroxisomes of methanol-grown Candida boidinii. Euron. J. Biochem. 59, 231–236 (1975)
Szabo, A.S., Avers, C.J.: Some aspects of regulation of peroxisomes and mitochondria in yeast. Ann. N.Y. Acad. Sci. 168, 302–312 (1969)
Tolbert, N.E.: Compartmentation and control in microbodies. Symp. Soc. exp. Biol. 27, 215–239 (1973)
Trelease, R.N., Becker, W.M., Gruber, P.J., Newcomb, E.H.: Microbodies (glyoxysomes and peroxisomes) in cucumber cytyledons. Plant Physiol. 48, 461–475 (1971)
Wergin, W.P.: Ultrastructural comparison of microbodies in pathogenic and saprophytic hyphae of Fusarium oxysporum f. sp. lycopersici. Phytopathology 62, 1045–1051 (1972)
Wergin, W.P.: Development of Woronin bodies from microbodies in Fusarium oxysporum f. sp. lycopersici. Protoplasma 76, 249–260 (1973)
Wilson, C.L., Stiers, D.L., Smith, G.G.: Fungal lysosomes or spherosomes. Phytopathology 60, 216–227 (1970)
About this article
Cite this article
Graves, L.B., Armentrout, V.N. & Maxwell, D.P. Distribution of glyoxylate-cycle enzymes between microbodies and mitochondria in Aspergillus tamarii . Planta 132, 143–148 (1976). https://doi.org/10.1007/BF00388895