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Planta

, Volume 127, Issue 1, pp 87–91 | Cite as

The hybridisation of [3H]poly(uridylic) acid to DNA from Phaseolus aureus Roxb.

  • Donald Grierson
Article

Summary

[5-3H]poly(uridylic) acid hybridises to nuclear DNA, suggesting the DNA contains poly (deoxyadenylic) acid sequences. These regions do not appear to be clustered in the DNA. Although the amount of DNA involved is small it is sufficient for thousands of sequences. Thermal dissociation of the hybrids shows that a relatively small number of hydrogen bonds is involved in each and it is concluded that these sequences are relatively short. In view of the fact that much longer poly(adenylic) acid sequences are found in plant RNA it is concluded that these are added after transcription.

Keywords

Hydrogen Hydrogen Bond Thermal Dissociation Longe Poly 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

A

adenine

T

thymine

G

guanine

C

cytosine

poly(A)

poly (adenylic) acid

poly(dA)

poly (deoxyadenylic) acid

poly(U)

poly (uridylic) acid

poly(dT)

poly (deoxythymidylic) acid

rRNA

ribosomal RNA

Tm

temperature at which 50% of a nucleic acid

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References

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Donald Grierson
    • 1
  1. 1.Department of Physiology and Environmental StudiesUniversity of Nottingham, School of AgricultureLoughboroughU.K.

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