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Planta

, Volume 115, Issue 1, pp 17–23 | Cite as

Cell wall assembly in vitro from Chlamydomonas reinhardi

  • G. J. Hills
Article

Summary

The conditions for in vitro dissociation and reassembly of the cell wall of Chlamydomonas reinhardi have been investigated. The cell wall dissociates in aqueous 8 M lithium chloride to two homogeneous components (6.8 S20w and 9.3 S20w)and dialysis of the cell wall subunits against water causes reassembly of a product having the same overall morphology and chemically similar to the original cell walls. Cell wall subunits in 8 M lithium chloride alone do not reassemble on dialysis against water to form cell walls but a nucleation centre has to be provided.

Keywords

Chloride Cell Wall Lithium Chlamydomonas Original Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • G. J. Hills
    • 1
  1. 1.John Innes InstituteNorwichU.K.

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