, Volume 115, Issue 1, pp 17–23 | Cite as

Cell wall assembly in vitro from Chlamydomonas reinhardi

  • G. J. Hills


The conditions for in vitro dissociation and reassembly of the cell wall of Chlamydomonas reinhardi have been investigated. The cell wall dissociates in aqueous 8 M lithium chloride to two homogeneous components (6.8 S20w and 9.3 S20w)and dialysis of the cell wall subunits against water causes reassembly of a product having the same overall morphology and chemically similar to the original cell walls. Cell wall subunits in 8 M lithium chloride alone do not reassemble on dialysis against water to form cell walls but a nucleation centre has to be provided.


Chloride Cell Wall Lithium Chlamydomonas Original Cell 
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  1. Ada, G.L., Norsal, G.J.V., Pye, J., Abbot, A.: Behaviour of active bacterial antigens during the induction of the immune response. I. Properties of flagellar antigens from Salmonella. Nature (Lond.) 199, 1275–1259 (1963)Google Scholar
  2. Boncroft, J.B., Hills, G.J., Markkam, R.: A study of the self assembly process in a small spherical virus. Virology 31, 354–379 (1967)CrossRefPubMedGoogle Scholar
  3. Borisy, G.G., Olmsted, J.B.: Nucleated assembly of microtubules in porcine brain extracts. Science 177, 1196–1197 (1972)PubMedGoogle Scholar
  4. Davies, D.R., Plaskitt, A.: Genetical and structural analyses of cell wall formation in Chalamydomonas reinhardi. Genet. Res. 17, 33–43 (1971)Google Scholar
  5. Hills, G.J., Gurney-Smith, M., Roberts, K.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardi. II. Electron microscopy and optical diffraction analysis. J. Ultrastruct. Res. 43, 179–192 (1973)PubMedGoogle Scholar
  6. Horne, R.W., Markham, R.: Practical methods in electron microscopy, vol. I., ed. Glauert, A. Amsterdam: North-Holland 1972Google Scholar
  7. Roberts, K., Gurney-Smith, M., Hills, G.J.: Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardi. I. Ultrastructure and preliminary chemical analysis. J. Ultrastruct. Res. 40, 599–613 (1972)PubMedGoogle Scholar
  8. Stephens, R.E.: Reassociation of microtubule protein. J. molec. Biol. 33, 517–519 (1968)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • G. J. Hills
    • 1
  1. 1.John Innes InstituteNorwichU.K.

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