, Volume 115, Issue 1, pp 1–10 | Cite as

Isoenzymes of acid phosphatase in germinating peas

  • C. B. Johnson
  • B. R. Holloway
  • H. Smith
  • D. Grierson


Acid phosphatase activity in pea cotyledons increases during germination. Gel filtration separates three isoenzymes with pH optima of 4.9, 5.6 and 6.0. The relative activities of these isoenzymes change during germination. The increase in activity is confined to two of the three isoenzymes. Density-labelling experiments whow that these are synthesised de novo. Activity of the third isoenzyme, which is present in dry seeds, does not change significantly during germination, and is not affected by cycloheximide but is lost on freezing. The possible localization of each isoenzyme is discussed in the light of these findings and in relation to histochemical studies.


Filtration Germinate Phosphatase Activity Acid Phosphatase Relative Activity 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • C. B. Johnson
    • 1
  • B. R. Holloway
    • 1
  • H. Smith
    • 1
  • D. Grierson
    • 1
  1. 1.Department of Physiology and Environmental StudiesUniversity of Nottingham, School of AgricultureLoughboroughU.K.

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