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The enzymic preparation of 14C-kaurene


Endosperm from immature seeds of Cucurbita pepo L. converts 2-14C-dl-mevalonate to 14C-(-)-kaurene with a yield of nearly 40% of the active isomer. Kaurene is the main product and the only diterpene hydrocarbon which is formed from mevalonate in the system and is therefore easily obtained radiochemically pure. The product was identified by thin-layer chromatography and recrystallization with authentic (-)-kaurene to constant specific radioactivity.

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Graebe, J.E. The enzymic preparation of 14C-kaurene. Planta 85, 171–174 (1969).

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  • Chromatography
  • Hydrocarbon
  • Recrystallization
  • Main Product
  • Enzymic Preparation