Endosperm from immature seeds of Cucurbita pepo L. converts 2-14C-dl-mevalonate to 14C-(-)-kaurene with a yield of nearly 40% of the active isomer. Kaurene is the main product and the only diterpene hydrocarbon which is formed from mevalonate in the system and is therefore easily obtained radiochemically pure. The product was identified by thin-layer chromatography and recrystallization with authentic (-)-kaurene to constant specific radioactivity.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Anderson, J. D., and T. C. Moore: Biosynthesis of (-)-kaurene in cell-free extracts of immature pea seeds. Plant Physiol. 42, 1527–1534 (1967).
Cross, B. E., R. H. B. Galt, and J. R. Hanson: The biosynthesis of the gibberellins. Part I. (-)-kaurene as a precursor of gibberellic acid. J. chem. Soc. 295–300 (1964).
Dennis, D. T., and C. A. West: Biosynthesis of Gibberellins. III. The conversion of (-)-kaurene to (-)-kauren-19-oic acid in endosperm of Echinocystis macrocarpa Greene. J. biol. Chem. 242, 3293–3300 (1967).
Graebe, J. E.: Biosynthesis of kaurene, squalene and phytoene from mevalonate-2-14C in a cell-free system from pea fruits. Phytochemistry 7, 2003–2020 (1968).
— D. T. Dennis, C. D. Upper, and C. A. West: Biosynthesis of Gibberellins. I. The biosynthesis of (-)-kaurene, (-)-kauren-19-ol, and trans-geranylgeraniol in endosperm nucellus of Echinocystis macrocarpa Greene. J. biol. Chem. 240, 1847–1854 (1965).
Hanson, J. R., and A. F. White: Studies in terpenoid biosynthesis — II. The biosynthesis of steviol. Phytochemistry 7, 595–597 (1968).
Jungalwala, F. B., and J. W. Porter: Biosynthesis of phytoene from isopentenyl and farnesyl pyrophosphates by a partially purified tomato enzyme system. Arch. Biochem. 119, 209–219 (1967).
Upper, C. D., and C. A. West: Biosynthesis of gibberellins. II. Enzymic cyclization of geranylgeranyl pyrophosphate to kaurene. J. biol. Chem. 242, 3285–3292 (1967).
West, C. A., M. Oster, D. Robinson, F. Lew, and P. Murphy: Biosynthesis of gibberellin precursors and related diterpenes. Paper presented at the Sixth Internat. Conf. Plant Growth Subst., Ottawa (1967).
About this article
Cite this article
Graebe, J.E. The enzymic preparation of 14C-kaurene. Planta 85, 171–174 (1969). https://doi.org/10.1007/BF00388547
- Main Product
- Enzymic Preparation