Human Genetics

, Volume 71, Issue 4, pp 325–328

Human ferritin light chain gene sequences mapped to several sorted chromosomes

  • R. V. Lebo
  • Y. W. Kan
  • Mei-Chi Cheung
  • S. K. Jain
  • J. Drysdale
Original Investigations
  • 40 Downloads

Summary

The iron storage ferritin light-chain gene exhibits multiple restriction enzyme fragments which have been mapped by analyzing sorted human chromosomes. A dual laser chromosome sorter was used to construct spot-blot filter panels representing 22 chromosome fractions. Hybridization of radiolabeled human ferritin-L gene probe to spot-blot panels revealed the ferritin-L gene on more than one chromosome. Miniaturized restriction enzyme analysis was used to map each of the ferritin-L restriction fragments uniquely to one of three chromosomes. This combination of sorted chromosome analyses provides a rapid method to map homologous DNA sequences located on more than one chromosome.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. V. Lebo
    • 1
  • Y. W. Kan
    • 1
  • Mei-Chi Cheung
    • 1
  • S. K. Jain
    • 2
  • J. Drysdale
    • 2
  1. 1.Howard Hughes Medical Institute and Department of MedicineUniversity of CaliforniaSan FranciscoUSA
  2. 2.Department of Biochemistry and PharmacologyTufts Medical SchoolBostonUSA

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