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Hormonal regulation of cotton ovule and fiber growth: Effects of bromodeoxyuridine, AMO-1618 and p-chlorophenoxyisobutyric acid

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Abstract

The effects of 5-bromo-2-deoxyuridine (BUdR, thymidine analogue), AMO-1618 (2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride), a growth retardant, and p-chlorophenoxyisobutyric acid (PCIB, an antiauxin) on growth (dry weight increase) and fiber development in unfertilized cotton (Gossypium hirsutum L.) ovules grown in vitro have been studied. BUdR (5 μM) causes about 70% inhibition of fiber production, with little effect on ovule growth, if applied during the first 6 d of culture in the presence of GA3 and IAA. AMO-1618, when used with GA3 alone, causes only a small reduction in both dry weight and fiber production, but when used with IAA alone reduces both fiber production and dry weight, the effect on the latter being predominant. In the presence of both IAA and GA3, AMO-1618 causes a small decrease in fiber production but a major decrease in dry weight. PCIB completely inhibits fiber growth but has little effect on dry weight, especially when GA3 is present. These results indicate that GA3 mainly promotes ovule growth while IAA is largerly responsible for fiber growth.

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Abbreviations

AMO-1618:

2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl chloride

BUdR:

5-bromo-2-deoxyuridine

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

PCIB:

p-chlorophenoxyisobutyric acid

TFU:

total fiber units

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Dhindsa, R.S. Hormonal regulation of cotton ovule and fiber growth: Effects of bromodeoxyuridine, AMO-1618 and p-chlorophenoxyisobutyric acid. Planta 141, 269–272 (1978). https://doi.org/10.1007/BF00388342

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Key words

  • AMO-1618
  • Antiauxin
  • Auxin
  • Bromodeoxyuridine
  • Cell (fiber) growth
  • Gibberellin
  • Gossypium
  • Ovules (in-vitro culture)