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Eine fördernde Wirkung von Blaulicht auf die Säureproduktion anaerob gehaltener Chlorellen

Blue-light-enhanced acid production of chlorella under anaerobiosis

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Summary

Under anaerobiosis the pH-value of the medium (0.002 M phosphate buffer) of a chlorophyll-free, carotenoid-containing mutant of Chlorella vulgaris (211-11h/20) drops slowly due to the excretion of acid fermentation end products. Blue light enhances this acidification of the medium (Figs. 1 and 2). Preliminary determinations of glycolic acid (color reaction with 2,7-dihydroxynaphthalene) indicate that there is about twice as much of this compound in the medium of an anaerobic culture kept in blue light as there is in the medium of one kept in the dark.

Addition of oxygen after a period of anaerobiosis in darkness or in blue light results in a greater O2-uptake by the previously illuminated cells (Fig. 3), indicating aerobic consumption of the acids released under nitrogen. The latter is proven by the experiment shown in Fig. 4, in which parallel cell samples develop a greater O2-consumption when suspended in the isolated media (phosphate buffer) of anaerobic cultures of the same organism instead of in fresh phosphate buffer, and a greater O2-consumption when suspended in the medium of an illuminated rather than in that of a dark anaerobic culture.

In experiments in which acid production is determined by measurement of the amount of 0.01 N NaOH required to keep the pH constant (Fig. 5), it can be shown that even traces of blue light can be effective in increasing the acidification of the medium of anaerobically kept cells; application of about 250 ergs cm-2 sec-1 of λ455 nm yields half-saturation (Fig. 6). Wavelengths around 470 and 370 nm are most effective in increasing this acid excretion; there is a minimum of activity around λ400 nm and no effect at all with yellow, red and far-red light (Fig. 7).

From the similarity between these intensity and spectral dependences and those for a light stimulation in respiration of the same organism found earlier (Kowallik, 1967), and from the fact that the acids released into the medium under anaerobiosis can be respired by the algae, we feel that both these increases are based on the same light reaction. The action of blue light in bringing about an enhancement in respiration might then consist in furnishing additional substrate.

Literatur

  1. Bassham, J. A.: Kinetic studies on the photosynthetic carbon reduction cycle. Ann. Rev. Plant Physiol. 15, 101–120 (1964).

  2. Egle, K., and H. Fock: Light respiration — correlations between CO2 fixation, O2 pressure, and glycollate concentration. In: Biochemistry of chloroplasts, ed. T. W. Goodwin, vol. 2, p. 79–87. London: Academic Press 1967.

  3. Hess, J. L. and N. E. Tolbert: Glycolate pathway in algae. Plant Physiol. 42, 371–379 (1967a).

  4. ——: Changes in chlorophyll a/b ratio and products of 14CO2 fixation by algae grown in blue or red light. Plant Physiol. 42, 1123–1130 (1967b).

  5. ——, and L. M. Pike: Glycolate biosynthesis by Scenedesmus and Chlorella in the presence or absence of NaHCO3. Planta (Berl.) 74, 278–285 (1967).

  6. Kowallik, U., u. W. Kowallik: Eine wellenlängenabhängige Atmungssteigerung während der Photosynthese von Chlorella. Planta (Berl.) 84, 141–157 (1969).

  7. Kowallik, W.: Über die Wirkung des blauen und roten Spektralbereichs auf die Zusammensetzung und Zellteilung synchronisierter Chlorellen. Planta (Berl.) 58, 337–365 (1962).

  8. —: Chlorophyll-independent photochemistry in algae. In: Energy conversion by the photosynthetic apparatus. Brookhaven Symp. Biol. 19, 467–477 (1966).

  9. —: Action spectrum for an enhancement of endogenous respiration by light in Chlorella. Plant Physiol. 42, 672–676 (1967).

  10. —: Der Einfluß von Licht auf die Atmung von Chlorella bei gehemmter Photosynthese. Planta (Berl.) 86, 50–62 (1969).

  11. —, and H. Gaffron: Respiration induced by blue light. Planta (Berl.) 69, 92–95 (1966).

  12. —— Enhancement of respiration and fermentation in algae by blue light. Nature (Lond.) 215, 1038–1040 (1967).

  13. Nelson, E. B., and N. E. Tolbert: Glycolate excretion and metabolism in unicellular green algae. Plant Physiol. 43, Suppl. S-12 (1968).

  14. Pätau, K.: Zur statistischen Beurteilung von Meßreihen (eine neue t-Tafel). Biol. Zbl. 63, 152–168 (1943).

  15. Poskuta, J.: Photosynthesis and respiration I. Effect of light quality on the photorespiration in attached shoots of spruce. Experientia (Basel) 24, 796–797 (1968).

  16. Stiller, M.: The path of carbon in photosynthesis. Ann. Rev. Plant Physiol. 13, 151–170 (1962).

  17. Syrett, P. J., and H.-A. Wong: The fermentation of glucose by Chlorella vulgaris. Biochem. J. 89, 308–315 (1963).

  18. Tolbert, N. E.: Glycolate pathway. In: Photosynthesis mechanism in green plants. Publ. 1145 Natl. Acad. Sci., Natl. Res. Counc. 648–662 (1963).

  19. Umbreit, W. W., R. H. Burris, and J. F. Stauffer: Manometric techniques and tissue metabolism. Minneapolis: Burgess Publ. Co. 1957.

  20. Urbach, W., and H. Gimmler: Stimulation of glycollate excretion of algae by disalicylidenepropanediamine and hydroxypyridinemethanesulfonate. Z. Naturforsch. 23b, 1282–1283 (1968).

  21. Wittingham, C. P., and G. G. Prichard: The production of glycollate during photosynthesis in Chlorella. Proc. roy. Soc. B. 157, 366–382 (1963).

  22. Wolf, V. J.: Nichtflüchtige Mono-, Di- und Tricarbonsäuren. In: Moderne Methoden der Pflanzenanalyse, ed. K. Paech und M. V. Tracey. Bd. 2, S. 478–582. Berlin-Göttingen-Heidelberg: Springer 1955.

  23. Zelitch, I.: The role of glycolic acid oxidase in the respiration of leaves. J. biol. Chem. 233, 1299–1303 (1958).

  24. —: Organic acids and respiration in photosynthetic tissues. Ann. Rev. Plant Physiol. 15, 121–142 (1964)

  25. —, and P. R. Day: Glycolate oxidase activity in algae. Plant Physiol. 43, 289–291 (1968a).

  26. ——: Variation in photorespiration. The effect of genetic differences in photorespiration on net photosynthesis in tobacco. Plant Physiol. 43, 1838–1844 (1968b).

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Kowallik, W. Eine fördernde Wirkung von Blaulicht auf die Säureproduktion anaerob gehaltener Chlorellen. Planta 87, 372–384 (1969). https://doi.org/10.1007/BF00388322

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