Planta

, Volume 120, Issue 1, pp 71–79 | Cite as

Transfer of algal chloroplasts into protoplasts of higher plants

  • Howard T. Bonnett
  • Tage Eriksson
Article

Summary

Polyethylene-glycol treatment of mixed suspensions of isolated protoplasts and of chloroplasts induces chloroplast uptake by the protoplasts. Chloroplasts of algal origin (Vaucheria dichotoma (L.) Ag.) were transferred with high frequency into protoplasts of Daucus carota L.

Keywords

Mixed Suspension Algal Origin Algal Chloroplast Chloroplast Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Carlson, P. S.: Towards a parasexual cycle in higher plants. In: Protoplasts et fusion de cellules somatiques végétales. Coll. Intern. C.N.R.S. No. 212, pp. 497–505 (1973a)Google Scholar
  2. Carlson, P. S.: The use of protoplasts for genetic research. Proc. nat. Acad. Sci. (Wash.) 70, 598–602 (1973b)Google Scholar
  3. Davey, M. R., Cocking, E. C.: Uptake of bacteria by isolated higher plant protoplasts. Nature (Lond.) 239, 455–456 (1972)Google Scholar
  4. Eriksson, T., Bonnett, H. T., Glimelius, K., Wallin, A.: Technical advances in protoplast isolation, culture and fusion. In: Advances in plant science through tissue culture, Street, H. E., ed. New York-London: Acad. Press, (in press) 1975Google Scholar
  5. Glimelius, K., Wallin, A., Eriksson, T.: Agglutinating effects of Concanavalin A on isolated protoplasts of Daucus carota. Physiol. Plant. 30, 564–569 (1974)Google Scholar
  6. Kao, K. N., Michayluk, M. R.: A method for high-frequency intergeneric fusion of plant protoplasts. Planta (Berl.) 115, 355–367 (1974)Google Scholar
  7. Nass, M. M. K.: Uptake of isolated chloroplasts by mammalian cells. Science 165, 1128–1131 (1969)PubMedGoogle Scholar
  8. Potrykus, I.: Transplantation of chloroplasts into protoplasts of Petunia. Z. Pflanzenphysiol. 70, 364–366 (1973)Google Scholar
  9. Potrykus, I., Hoffman, F.: Transplantation of nuclei into protoplasts of higher plants. Z. Pflanzenphysiol. 69, 287–289 (1973)Google Scholar
  10. Trench, R. K., Gooday, G. W.: Incorporation of [3H]-leucine into protein by animal tissues and by endosymbiotic chloroplasts in Elysia viridis Montagu. Comp. biochem. Physiol. 44A, 321–330 (1973)CrossRefGoogle Scholar
  11. Wallin, A., Eriksson, T.: Protoplast cultures from cell suspensions of Daucus carota. Physiol. Plant. 28, 33–39 (1973)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Howard T. Bonnett
    • 1
  • Tage Eriksson
    • 1
  1. 1.Institute of Physiological BotanyUniversity of UppsalaUppsalaSweden

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