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Uptake and reduction of glycerate by isolated chloroplasts

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Summary

Intact chloroplasts of spinach (Spinacia oleracea L.) evolve O2 in the light in a glycerate-dependent reaction at rates usually close to 10 μmolxmg-1 chlorophyllxh-1. Glycerate isfirst phosphorylated and the resulting phosphoglycerate reduced to the sugar level. Products of the reaction are the intermediates of the Calvin cycle and glycolate. The ratio of triosephosphates to phosphoglycerate is higher under low light or at a low pH than under high light or at a high pH. Chloroplasts contain activities of glycerate kinase which approximately correspond to observed glycerate reduction rates at light saturation. The main part of the glycerate kinase of leaf cells is localized in the chloroplasts, but considerable activity also resides outside these organelles. Glycerate can enter intact chloroplasts of spinach as the anion and the undissociated acid. It can thus mediate indirect proton transfer across the chloroplast envelope. In the presence of slowly permeating cations it is taken up mainly in an anion exchange reaction. Chloride and acetate anions permeate faster than the glycerate anion. The relation between glycerate reduction and photorespiration is discussed.

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Abbreviations

PGA:

phosphoglycerate

DHAP:

dihydroxyacetone phosphate

HMP:

hexose and heptose monophosphates

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea, chl chlorophyll, E enzyme

References

  1. Bassham, J. A., Kirk, M., Jensen, R. G.: Photosynthesis by isolated chloroplasts. I. Diffusion of labelled photosynthetic intermediates between isolated chloroplasts and suspending medium. Biochim. biophys. Acta (Amst.) 153, 211–218 (1968)

  2. Björkman, O.: The effect of oxygen concentration on photosynthesis in higher plants. Physiol. Plant. 19, 618–633 (1966)

  3. Black, C. C. jr.: Photosynthetic carbon fixation in relation to net CO2 uptake. Ann. Rev. Plant Physiol. 24, 253–286 (1973)

  4. Chan, H. W. S., Bassham, J. A.: Metabolism of 14C-labelled glycolic acid by isolated spinach chloroplasts. Biochim. biophys. Acta (Amst.) 141, 426–429 (1967)

  5. Coombs, J., Whittingham, C. P.: The effect of high partial pressure of oxygen on photosynthesis in Chlorella. Phytochemistry 5, 643–651 (1966)

  6. Gimmler, H., Schäfer, G., Kraminer, H., Heber, U.: Amino acid permeability of the chloroplast envelope as measured by light scattering, volumetry and amino acid uptake. Planta (Berl.) 120, 47–61 (1974)

  7. Heber, U.: Stoichiometry of reduction and phosphorylation during illumination of intact chloroplasts. Biochim. biophys. Acta (Amst.) 305, 140–152 (1973)

  8. Heber, U.: Metabolite exchange between chloroplasts and cytoplasm. Ann. Rev. Plant Physiol. 25, 393–421 (1974)

  9. Heber, U., Krause, G.: Hydrogen and proton transfer across the chloroplast envelope. Proc. IInd Inter. Congr. Photosynthesis Res. vol. II, p. 1023–1033, Forti, G., Avron, M., Melandri, A. eds. The Hague: W. Junk 1972

  10. Heber, U., Pon, N. G., Heber, M.: Localization of carboxydismutase and triosephosphate dehydrogenases in chloroplasts. Plant Physiol. 38, 355–360 (1963)

  11. Heber, U., Santarius, K. A.: Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforsch. 25b, 718–728 (1970)

  12. Heber, U., Willenbrink, J.: Sites of synthesis and transport of photosynthetic products within the leaf cell. Biochim. biophys. Acta (Amst.) 82, 313–324 (1964)

  13. Heldt, H. W., Rapley, L.: Specific transport of inorganic phosphate 3-phosphoglycerate and dihydroxyacetone phosphate, and of dicarboxylic acid anions in spinach chloroplasts. FEBS-Letters 10, 143–148 (1970)

  14. Heldt, H. W., Sauer, F.: The inner membrane of the chloroplast envelope as the site of specific metabolite transport. Biochim. biophys. Acta (Amst.) 234, 83–91 (1971)

  15. Heldt, H. W., Werdan, K., Milovancev, M., Geller, G.: Alkalization of the chloroplast stroma caused by light-dependent proton flux into the thylakoid space. Biochim. biophys. Acta (Amst.) 314, 224–241 (1971)

  16. Jackson, W. A., Volk, R. J.: Photorespiration. Ann. Rev. Plant Physiol. 21, 385–432 (1970)

  17. Jensen, R. G., Bassham, J. A.: Photosynthesis by isolated chloroplasts. Proc. nat. Acad. Sci. (Wash.) 56, 1095–1101 (1966)

  18. Klingenberg, M.: Mitochondrial metabolite transport. FEBS-Letters 6, 145–154 (1970)

  19. Klingenberg, M., Pfaff, E.: Means of terminating reactions. In: Methods of enzymology (Colowick, S. P., Kaplan, N. I., eds.), vol. X, p. 680–684. New York: Acad. Press 1967

  20. Packer, L., Crofts, A. R.: The energized movements of ions and water by chloroplasts. In: Current topics in bioenergetics (Sanadi, D. R., ed.), vol. II, p. 24–62. New York: Acad. Press 1967

  21. Pedersen, T. A., Kirk, M., Bassham, J. A.: Inhibition of photophosphorylation and photosynthetic carbon cycle reactions by fatty acids and esters. Biochim. biophys. Acta (Amst.) 112, 189–203 (1966)

  22. Pressman, B. C.: Alkali metal chelators—The ionophores. In: Inorganic Biochemistry (Eichhorn, G. L. ed.), vol. I, p. 203–226. Amsterdam: Elsevier, 1973

  23. Santarius, K. A., Stocking, C. R.: Intracellular localization of enzymes in leaves and chloroplast membrane permeability to compounds involved in amino acid synthesis. Z. Naturforsch. 24b, 1170–1179 (1969)

  24. Stokes, D. M., Walker, D. A.: Relative impermeability of the chloroplast envelope to ATP. In: Photosynthesis and photorespiration (Hatch, M. D., Osmond, C. G., Slatyer, R. O. eds.), p. 226–231. New York: Wiley Interscience 1971

  25. Tolbert, N. E.: Microbodies—peroxisomes and glycosomes. Ann. Rev. Plant. Physiol. 22, 45–74 (1971)

  26. Tolbert, N. E., Zill, L. P.: Excretion of glycolic acid by algae during photosynthesis. J. biol. Chem. 222, 895–906 (1956)

  27. Walker, D. A.: Chloroplast and Cell—Concerning the movement of certain key metabolites etc. across the chloroplast envelope. In: MTB Intern. Rev. Science Biochem. Sect. (Northcote, D. H. ed.), vol. 11, in press (1974)

  28. Walker, D. A., Kosciukiewicz, K., Case, C.: Photosynthesis by isolated chloroplasts: Some factors affecting induction in CO2-dependent oxygen evolution. New Phytol. 72, 237–247 (1973)

  29. Zelitch, I.: Organic acids and respiration in photosynthetic tissues. Ann. Rev. Plant Physiol. 15, 121–142 (1964)

  30. Zelitch, I.: Alternate pathways of glycolate synthesis in Tobacco and Maize leaves in relation to the rate of photorespiration. Plant Physiol. 51, 299–305 (1973)

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Heber, U., Kirk, M.R., Gimmler, H. et al. Uptake and reduction of glycerate by isolated chloroplasts. Planta 120, 31–46 (1974). https://doi.org/10.1007/BF00388269

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Keywords

  • Anion Exchange
  • Reduction Rate
  • Proton Transfer
  • High Light
  • Phosphoglycerate