, Volume 137, Issue 3, pp 287–291 | Cite as

Rosmarinic acid production in Coleus cell cultures

  • A. Razzaque
  • B. E. Ellis


Cell suspension cultures of Coleus blumei Benth. have been found to accumulate 8–11% of their dry weight as rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenyl-lactic acid). Actively-growing tissue converts >20% of exogenously supplied phenylalanine and tyrosine to the caffeoyl ester and this high rate of synthesis coincides with an increase in phenylalanine ammonia-lyase specific activity. Administration to the cultures of known phenylpropanoid precursors of rosmarinic acid failed to enhance the latter's production and in some cases inhibited it.

Key words

Cell culture Coleus Rosmarinic acid 



rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenyllactic acid




phenylalanine ammonialyase


dihydroxyphenyl-lactic acid


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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • A. Razzaque
    • 1
  • B. E. Ellis
    • 1
  1. 1.Guelph-Waterloo Centre for Graduate Work in ChemistryUniversity of GuelphGuelphCanada

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