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Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L.

I. Separation and characterisation

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The preparation is described of a cell-free system from developing cotyledons of Phaseolus vulgaris cv. Canadian Wonder which is capable of binding ethylene. The binding is saturable and the apparent dissociation constant for ethylene is 6.4·10-10 M in solution. The binding site is associated with subcellular particles and treatment with Triton X-100 results in substantial solubilisation of the activity. The kinetics of association and dissociation of the ligand and the binding site are described. The system is heat labile and binding activity is diminished by treatment with some proteolytic enzymes.

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N-2-hydroxyethylpiperazine propane sulfonic acid


  1. Batt, S., Wilkins, M.B., Venis, M.A.: Auxin binding to corn coleoptile membranes: kinetics and specificity. Planta 130, 7–13 (1976)

  2. Bengochea, T., Acaster, M.A., Dodds, J.H., Evans, D.E., Jerie, P.H., Hall, M.A.: Studies on ethylene binding by cell free preparations from cotyledons of Phaseolus vulgaris L. II. Effects of structural analogues of ethylene and of inhibitors. Planta 148, 407–411 (1980)

  3. Best-Belpomme, M., Mester, J., Weintraub, H., Baulieu, E.E.: Oestrogen receptors in chick oviduct. Characterisation and subcellular distribution. Eur. J. Biochem. 57, 537–549 (1975)

  4. Beyer, E.M., Jr.: [14C]Ethylene incorporation and metabolism in pea seedlings. Nature (London) 255, 144–147 (1975)

  5. Beyer, E.M. Jr.: Silver ion: A potent antiethylene agent in cucumber and tomato. Hortic. Sci. 11 (3), 195–196 (1976)

  6. Beyer, E.M. Jr., Blomstrom, D.C.: Ethylene metabolism and its possible physiological role in plants. Proc. 10th International Conference on Plant Growth Substances (in press)

  7. Chang, K.-J., Jacobs, S., Cuatrecasas, P.: Quantitative aspects of hormone-receptor interactions of high affinity. Effect of receptor concentration and measurement of dissociation constants of labelled and unlabelled hormones. Biochim. Biophys. Acta. 406, 294–303 (1975)

  8. Cheng, K.-W.: Properties of follicle-stimulating-hormone receptor in cell membranes of bovine testis. Biochem. J. 149, 123–132 (1975)

  9. Dodds, J.H., Musa, S.K., Jerie, P.H., Hall, M.A.: Metabolism of ethylene to ethylene oxide by cell-free preparations from Vicia faba. Plant. Sci. Lett. 17, 109–114 (1979)

  10. Gibson, M.S.: Incorporation of pyruvate[C14) into ethylene by Penicillium digitatum Sacc. Arch. Biochem. Biophys. 106, 312–316 (1964)

  11. Hall, M.A., Acaster, M.A., Bengochea, T., Dodds, J.H., Evans, D.E., Jones, J.F., Jerie, P.H., Mutumba, G.C., Niepel, B., Shaari, A.R.: Ethylene and seeds. Proc. 10th International Conference on Plant Growth Substances (in press)

  12. Hall, M.A., Kapuya, J.A., Sivakumaran, S., John, A.: The role of ethylene in the response of plants to stress. Pestic. Sci. 8, 217–223 (1977)

  13. Hertel, R., Thomson, K., Russo, V.E.A.: In vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta 107, 325–340 (1972)

  14. Hollenberg, M.D., Cuatrecasas, P.: Studies on the interaction of hormones with plasma membrane receptors In: Biochemical Actions of Hormones, vol 3, pp. 41–85. Litwach, G. ed. New York: Academic Press 1975

  15. Jerie, P.H., Hall, M.A.: The identification of ethylene oxide as a major metabolite of ethylene in Vicia faba L. Proc. R. Soc. London Ser. B 200, 87–94 (1978)

  16. Jerie, P.H., Shaari, A.R., Hall, M.A.: The compartmentation of ethylene in developing cotyledons of Phaseolus vulgaris L. Planta 144, 503–507 (1979)

  17. Jerie, P.H., Shaari, A.R., Zeroni, M., Hall, M.A.: The partition coefficient of 14C2H4 in plant tissue as a screening test for metabolism or compartmentation of ethylene. New Phytol. 81, 499–504 (1978)

  18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)

  19. Pohl, S.L.: Glucagon receptor. In: The Role of Membranes in Metabolic Regulation, pp. 349–365. Mehlman, M.A., Hanson, R.W. eds. New York: Academic Press 1972

  20. Ray, P.M., Dohrmann, U., Hertel, R.: Characterisation of naphthaleneacetic acid binding to receptor sites on cellular membranes of maize coleoptile tissue. Plant Physiol. 59, 357–364 (1977)

  21. Scatchard, G.: The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. 51, 660–672 (1949)

  22. Sisler, E.C., Filka, M.E.: Partial purification of ethylene binding component from plant tissue. Plant Physiol. 63, 5–131 (1979)

  23. Venis, M.A.: Receptors for plant hormones. In: Adv. Bot. Res. 5, 53–87. Woolhouse, H.W. ed. London: Academic Press 1977

  24. Venis, M.A., Watson, P.J.: Naturally occurring modifiers of auxinreceptor interaction in corn: Identification as benzoxazolinones. Planta 142, 103–107 (1978)

  25. Young, R.E., Pratt, H.K., Biale, J.B.: Manometric determination of low concentrations of ethylene. Anal. Chem. 24, 551–555 (1952)

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Bengochea, T., Dodds, J.H., Evans, D.E. et al. Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgaris L.. Planta 148, 397–406 (1980). https://doi.org/10.1007/BF00388129

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Key words

  • Ethylene binding
  • Hormone binding
  • Phaseolus